African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12196

Full Length Research Paper

Complete genomic sequence and recombination analysis of wheat yellow mosaic virus isolate from Zhouzhi in China

Zong-Ying Zhang
  • Zong-Ying Zhang
  • Department of Plant Pathology and State Key Laboratory for Agrobiotechnology, China Agriculture University, 100193 Beijing, China.
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Cui-Ji Zhou
  • Cui-Ji Zhou
  • Department of Plant Pathology and State Key Laboratory for Agrobiotechnology, China Agriculture University, 100193 Beijing, China.
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Yun-Feng Wu
  • Yun-Feng Wu
  • Department of Plant Pathology, Northwest A&F University, 712100 Yangling, China.
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Da-Wei Li
  • Da-Wei Li
  • Department of Plant Pathology and State Key Laboratory for Agrobiotechnology, China Agriculture University, 100193 Beijing, China.
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Jia-Lin Yu
  • Jia-Lin Yu
  • Department of Plant Pathology and State Key Laboratory for Agrobiotechnology, China Agriculture University, 100193 Beijing, China.
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Cheng-Gui Han
  • Cheng-Gui Han
  • Department of Plant Pathology and State Key Laboratory for Agrobiotechnology, China Agriculture University, 100193 Beijing, China.
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  •  Received: 15 June 2018
  •  Accepted: 21 November 2018
  •  Published: 03 April 2019

Abstract

Wheat yellow mosaic virus (WYMV) is the causal agent of wheat yellow mosaic disease in China. WYMV was detected in wheat sample collected from Zhouzhi of Shanxi province. The nearly complete genomic sequence of Zhouzhi isolate (WYMV-ZZ) was determined; it was compared with six complete sequences of WYMV isolates (five Chinese isolates and one Japanese isolate). WYMV-ZZ and the other six different WYMV isolates shared 96.6 to 97.7% and 95.1 to 98.2% nucleotide sequence identity for RNA1 and RNA2, respectively; at the amino acid level, WYMV-ZZ had 94.1 to 98.2% identity for RNA1 and 94.1 to 96.7% identity for RNA2, respectively, with the other six isolates. Phylogenetic analysis showed that the NIa-VPg region can separate the Chinese isolates from Japanese isolate. Based on the recombinant analysis, there were three possible recombination events; one event was located in RNA1 CI region of WYMV-ZZ with a RDP P-value of 8.526×10-06. This work advanced our understanding of the WYMV molecular variation and was helpful to study the disease spread.

 

Key words: Sequence comparison, phylogenetic analysis, NIa-VPg, recombinant event.

 

Abbreviation

WYMV, Wheat yellow  mosaic  virus;  WYMV-HC,  WYMV isolate of Huangchuan, Henan province; WYMV-YZ, WYMV isolate of Yangzhou, Jiangsu province; WYMV-YA, WYMV isolate of Ya’an, Sichuan province; WYMV-ZMD, WYMV isolate of Zhumadian, Henan province; WYMV-XQ, WYMV isolate of Xiaqiao, Jiangsu province; WYMV-JPN, WYMV isolate of Japan; CP, coat protein; VPg, viral genome-linked protein.