African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

In vitro multiple shoot bud induction and regeneration from plumule junction explants of pigeon pea [Cajanus cajan (L.) Mill sp.] cultivars

Vandana Kashyap
  • Vandana Kashyap
  • Department of Biotechnology, D.D.U Gorakhpur University, Gorakhpur, Uttar Pradesh- 273 009, India.
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Bijaya K Sarangi
  • Bijaya K Sarangi
  • Environmental Biotechnology Division, National Environmental Engineering research Institute- CSIR, Nehru Marg, Nagpur-440 020, India.
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Manoj K Yadav
  • Manoj K Yadav
  • Department of Biotechnology, College of Agriculture, S.V.P university of Agriculture and Technology, Meerut 250 110, India.
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Dinesh Yadav*
  • Dinesh Yadav*
  • Department of Biotechnology, D.D.U Gorakhpur University, Gorakhpur, Uttar Pradesh- 273 009, India.
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  •  Received: 12 February 2014
  •  Accepted: 29 September 2014
  •  Published: 08 October 2014

Abstract

The response of eleven Indian cultivars of pigeon pea for in vitro multiple shoot bud induction and regeneration from plumule junction explants under variable concentration of 6-benzyl amino purine (BAP), kinetin and thiadiazuron (TDZ) was assessed in the present study. The cultivar IPA-3088 showed best response with a maximum of 20 buds per explants in Murashige and Skoog (MS) media supplemented with 0.05 mgL-1 TDZ. Among the hormones tested, lower concentration of TDZ gave the best response for these cultivars though higher concentration of BAP was also effective in multiple shoot bud induction and regeneration from plumule junction explants. The elongation of multiple shoot buds was achieved in the same medium and the nature of the regenerants in most of the cases was shoots, though in few cases shoot buds and shoot primoridia were also observed. Rooting of plumule junction derived shootlets was found to be better in the presence of NAA as compared to IAA and IBA for most of the cultivars producing maximum number of primary roots. The best responding cultivar IPA 3088 showed efficient rooting in the presence of 0.2 mgL-1 of NAA. The regenerated plantlets were acclimatized in soil with percentage of acclimatization varying from 40-80% for different cultivars.

 

Key words: Pigeon pea, cultivars, multiple shoot bud induction, organogenesis, acclimatization, elongation, Cajanus cajan (L) Mill sp.