African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Full Length Research Paper

Peroxidase and polyphenol oxidase activities associated to somatic embryogenesis potential in an elite hybrid genotype of Theobroma cacao L.

Minyaka Emile
  • Minyaka Emile
  • Biochemistry Laboratory, Faculty of Science, University of Douala, P. O. Box 24157 Douala, Cameroon.
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Niemenak Nicolas
  • Niemenak Nicolas
  • Laboratory of Plant Physiology and Biochemistry, Department of Biological Science, Higher Teachers Training, College of Yaoundé, P.O. Box 47 Yaoundé, Cameroon.
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Ngangue Latta Tanguy Armand
  • Ngangue Latta Tanguy Armand
  • Biochemistry Laboratory, Faculty of Science, University of Douala, P. O. Box 24157 Douala, Cameroon.
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Madina Banen Colette Vanessa
  • Madina Banen Colette Vanessa
  • Biochemistry Laboratory, Faculty of Science, University of Douala, P. O. Box 24157 Douala, Cameroon.
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Bahoya Joseph Aime
  • Bahoya Joseph Aime
  • Biochemistry Laboratory, Faculty of Science, University of Douala, P. O. Box 24157 Douala, Cameroon.
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Omokolo Ndoumou Denis
  • Omokolo Ndoumou Denis
  • Laboratory of Plant Physiology and Biochemistry, Department of Biological Science, Higher Teachers Training, College of Yaoundé, P.O. Box 47 Yaoundé, Cameroon.
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  •  Received: 18 July 2017
  •  Accepted: 16 November 2017
  •  Published: 06 December 2017

Abstract

Cross-fertilization and somatic embryogenesis stand as potential useful tools for genetic improvement and vulgarization of agronomical interesting cocoa planting material for sustainable cocoa culture. Manual cross-fertilization (♀SNK13×♂UPA143) was conducted. ♀SNK13×♂UPA143 derived hybrid genotypes were tested for their susceptibility to black pods disease (BPD). The most tolerant hybrid genotype (KHACa12) was monitored for its precocity, yielding and somatic embryo potential. Peroxidase and polyphenol oxidase activities were evaluated in association to embryogenesis in KHACa12. The cross-fertilization ♀SNK13×♂UPA143 generated around 60% of offprint’s tolerant or less susceptible to BPD. When transferred in experimental farm, KHACa12 produced flowers 18 months after planting. Mature pods from KHACa12 bearded 55±5 seeds/pod. Seventy-five pods were harvested from KHACa12 during the first season. These pods generated 6 kg of fermented-dried cocoa seeds. Somatic embryo responsive revealed that 71.041 ± 7.91% and 50.64 ± 9.48% staminodes and petals derived explants, respectively produced direct and indirect somatic embryos. Morphologically, the somatic embryos obtained were similar to zygotic embryos from the same hybrid genotype (KHACa12). Peroxidase and polyphenol oxidase activities profiles in zygotic and somatic embryos increase during the first stages of embryogenesis, then decrease when cotyledons developed. These results might indicate that, ♀SNK13×♂UPA143 could be used to develop cocoa (Theobroma cacao L.) hybrid genotypes tolerant or less susceptible to BPD. KHACa12 is an agronomic interesting hybrid genotype that could be produced in large scale and vulgarized using somatic embryogenesis process. The initiation and development of somatic embryo in KHACa12 can be monitored and modulated biochemically through peroxidase and polyphenol oxidase activities profiles.

Key words: Breeding, tissue culture, somatic, explants, hybrid.