African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12193

Full Length Research Paper

Differential expression of ozone-induced gene during exposures to salt stress in Polygonum sibiricum Laxm leaves, stem and underground stem

Shou-Hai Na1, Chun-Pu Qu2, Guan-Jun Liu2*, Chun Liu2, Rui-Xuan Gu2, Zhi-Gang Wei2, Gui-Feng Liu2 and Chuan-Ping Yang2
1College of Wildlife Sciences, Northeast Forestry University, Harbin 150040, P. R. China. 2The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
Email: [email protected]

  •  Accepted: 12 May 2010
  •  Published: 31 August 2010

Abstract

The response of plants to environmental stresses is characterized by a number of physiological and biochemical changes that ultimately result from the selective increase or decrease in the biosynthesis of a large number of distinct proteins. In this report, we describe the characterization of an ozone-induced transcript, PcOZI-1, which has been identified from Polygonum sibiricum Laxm by the rapid amplification of cDNA ends method (RACE). PcOZI-1 mRNA in untreated plants was detected at low levels in underground stem, leaves and at higher levels in stem. PcOZI-1 mRNA accumulation was transiently induced in stem 7-fold within the first 8 h of 3% NaHCO3 treatment. PcOZI-1 mRNA accumulation was also induced 22-fold in underground stem after 72 h. Sequence analysis of PcOZI-1revealed that it encodes a 8.45 kDa basic protein that contains a putative signal peptide. Our results suggest that PcOZI-1 represents a novel stress-related protein that accumulates in response to the production of active oxygen species.

 

Key words: Polygonum sibiricum Laxm, PcOZI-1, rapid amplification of cDNA ends, gene expression.

Abbreviation

OZI, Ozone-induced gene; EST, expressed sequence tag; cDNA,complementary DNA; RACE, Rapid amplification of cDNA ends; PcOZI-1,Polygonum sibiricum Laxm ozone-induced gene transcript; PCR, polymerase chain reaction; RT-PCR, reverse transcription-PCR; ORF, open reading frame; SDS,sodium dodecyl sulfate; UTR, 5'-untranslated region.