African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12240

Full Length Research Paper

Comparison of seed priming techniques with regards to germination and growth of watermelon seedlings in laboratory condition

Willams Ferreira Souza Barbosa
  • Willams Ferreira Souza Barbosa
  • Mato Grosso do Sul State University - UEMS, Department of Agronomy, Rodovia MS 306, km 6.4, Cassilândia, Mato Grosso do Sul, Brazil.
  • Google Scholar
Fábio Steiner
  • Fábio Steiner
  • Mato Grosso do Sul State University - UEMS, Department of Agronomy, Rodovia MS 306, km 6.4, Cassilândia, Mato Grosso do Sul, Brazil.
  • Google Scholar
Leandro Castro Matias de Oliveira
  • Leandro Castro Matias de Oliveira
  • Mato Grosso do Sul State University - UEMS, Department of Agronomy, Rodovia MS 306, km 6.4, Cassilândia, Mato Grosso do Sul, Brazil.
  • Google Scholar
Paulo Henrique
  • Paulo Henrique
  • Mato Grosso do Sul State University - UEMS, Department of Agronomy, Rodovia MS 306, km 6.4, Cassilândia, Mato Grosso do Sul, Brazil.
  • Google Scholar
Menezes das Chagas
  • Menezes das Chagas
  • Mato Grosso do Sul State University - UEMS, Department of Agronomy, Rodovia MS 306, km 6.4, Cassilândia, Mato Grosso do Sul, Brazil.
  • Google Scholar


  •  Received: 15 February 2016
  •  Accepted: 11 November 2016
  •  Published: 16 November 2016

Abstract

Seeds of watermelon [Citrullus lanatus (Thunb.) Matsum and Nakai, cv. Crimson Sweet] were used to investigate the effects of different priming techniques on seed germination and early seedling growth. The seeds were soaked in solutions of 0.2% gibberellin (50 mg L–1 gibberellic acid), 0.2% cytokinin (90 mg L–1 kinetin), 0.2% potassium nitrate (2 g L–1 KNO3), 0.2% calcium nitrate [2 g L–1 Ca(NO3)2] or water (hydropriming) for 6 h at 25°C. After drying, five replicates of 25 seeds were distributed in plastic boxes with blotter paper and kept into a seed germinator at 26°C for 12 days. The different priming treatments significantly affect the measurements of the seed germination and growth of watermelon seedlings. The germination of watermelon seeds ranged from 0 to 100%, and was significantly greater when seeds were subjected to priming with GA3, KNO3, Ca(NO3)2 and water (control), and lower under cytokinin (CK) priming. The seed priming with 0.2% solution of CK resulted in 100% of abnormal seedlings, and therefore should not be used by watermelon growers. Seed priming with GA3, KNO3, Ca(NO3)2 and water (hydropriming) increased the shoot length, whereas GA3, Ca(NO3)2 and water priming improved the radicle length, as well as shoot dry matter watermelon seedlings. The KNO3 and water priming increased the root dry matter of watermelon seedlings. Seed priming with KNO3 and Ca(NO3)2 improved the germination rate and seedling vigor index. The results of this study show that seed priming with GA3, KNO3, Ca(NO3)2 and water (hydropriming) may be useful tools due to their positive effects on germination percentage and growth characteristics of watermelon seedlings.

Key words: Citrullus lanatus, cytokinin, gibberellin, hydropriming.