African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Full Length Research Paper

The influence of endogenous hormones on the formation of buds from stems of bitter melon (Momordıca charantıa L.)

Yi Tang, Ji Liu, Juan Li, Xiao-Mei Li, Bin Liu and Huan-Xiu Li*      
College of Horticulture, Sichuan Agricultural University, Ya’an 625014, Sichuan, People’s Republic of China.  
Email: [email protected]

  •  Accepted: 02 June 2011
  •  Published: 29 June 2011

Abstract

Stems of bitter melon (Momordica charantia L.) cv. Dabai were used to establish in vitro cultures. The endogenous hormone concentrations (indoleacetic acid [IAA], abscisic acid [ABA], gibberellins 3 [GA3], zeatin [ZT]) of the calluses were determined by means of high pressure liquid chromatography (HPLC). The endogenous ZT was higher in the stem calluses that had formed buds, and there was a higher IAA/ZT ratio and GA3/ZT ratio in the calluses having no capacity for buds formation. The results showed that addition of plant growth regulator influences endogenous hormone status and it will be helpful for in vitro propagation of bitter melon.

 

Key words: Endogenous phytohormones, high pressure liquid chromatography, in vitro culture, bitter melon (Momordica charantia L.).

Abbreviation

ABA, Abscisic acid; B, calluses that had formed buds; BA,benzyladenine; 2,4-D, 2,4-dichlorophenoxyacetic acid; FW, fresh weight; GA3,gibberellins 3; HPLC, high pressure liquid chromatography; IAA, indole acetic acid;MS, Murashige and Skoog (1962); NB, calluses that had no bud formation capacity;ZT, zeatin.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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