African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12337

Full Length Research Paper

Effect of interaction of 6-benzyl aminopurine (BA) and sucrose for efficient microtuberization of two elite potato (Solanum tuberosum L.) cultivars, Desiree and Cardinal

Aafia Aslam1*, Aamir Ali2, Naima Huma Naveed2, Asif Saleem2 and Javed Iqbal1  
1Seed Centre, University of the Punjab, Quaid-e-Azam Campus, 54590, Lahore, Pakistan. 2Department of Biological Sciences, University of Sargodha, Sargodha, Pakistan.  
Email: [email protected]

  •  Accepted: 01 September 2011
  •  Published: 03 October 2011

Abstract

Single node and multinode explants of two potato cvs., Desiree and Cardinal were tested for in vitro microtuber production. Explants were taken from tissue culture laboratory of Seed Centre, University of the Punjab, Lahore, Pakistan in 2004. Experiments were designed in completely randomized pattern. 34 media treatments of Murashige and Skoog (1962) with varying concentrations of sucrose (4, 6, 8, 10 and 12%) either alone or in combination (5-8% sucrose) with 6-benzyl amino purine (BA) were studied. In the case of cv. Desiree, medium MB10 (BA 6 mg/l, sucrose 6%) and for cv. Cardinal, medium MK20 (BA 5 mg/l, sucrose 8%) in term of induction, mean number and mean fresh weight per single node explant were optimized. In comparison, cv. Desiree genotypically was observed to be slightly slow in response to in vitro microtuber induction and development than cv. Cardinal.

 

Key words: Potato, microtuberproduction, 6-benzyl aminopurine (BA), sucrose.

 

Abbreviation

 BA, 6-Benzyl aminopurineMS, Murashige and Skoog.