African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12306

Full Length Research Paper

Analysis of alkaloid phytochemical compounds in the ethanolic extract of Datura stramonium and evaluation of antimicrobial activity

Huda Jasim Altameme
  • Huda Jasim Altameme
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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Imad Hadi Hameed*
  • Imad Hadi Hameed*
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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Muhanned Abdulhasan Kareem
  • Muhanned Abdulhasan Kareem
  • Babylon University, Centre of Environmental Research, Iraq.
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  •  Received: 02 March 2015
  •  Accepted: 06 May 2015
  •  Published: 13 May 2015


The aim of this study was to assess the compounds of alkaloids extracts from the leaves of Datura stramonium, which can be the basis for the synthesis of new antibiotics. In this study, the alkaloid compounds of D. stramonium have been evaluated. The chemical compositions of the leaves of ethanolic extract of D. stramonium were investigated using gas chromatography-mass specroscopy. Gc-MS analysis of alkaloid leaves ethanolic extract of D. stramonium revealed the existence of the Ethyl iso-allocholate, D-asycarpidan-1-methanol, acetate (ester), 3-(1,5-dimethyl-hexyl)3a,10,10,12b-tetramethyl1,2,3,3a,4,6,8,9,10,10a,11,12,12a,12b-tetradec- ahydro -benzo[4,5] cyclohept,2,7-Diphenyl-1,6-dioxopyridazino [4,5:2,3] pyrrolo[4,5-d] pyridazine, 3,8,8-Trimethoxy-3-piperidyl-2,2-benaphthalene-1,1,4,4-tetrone, [5β] Pregnane3, 20β-diol,14α,18α-[4-methyl,3-oxo-[1-oxa-4-azabutane-1,4-diyl], diacetate, 1-monolinoleoylglycerol trimethylsilyl ether and 17-[1,5-dimethylhexyl]-10,13-dimethyl-3sstyrylhexadecahydrocyclopenta[a]phenathren-2-one. Alkaloids extract from leaves of D. stramonium were assayed for in vitro antibacterial activity against Escherichia coli, Proteus mirabilis, Staphylococcus aureus, Pseudomonas aerogenosa and Klebsiella pneumonia by using the diffusion method in agar. The zone of inhibition was compared with different standard antibiotics. The diameters of inhibition zones ranged from 0.8 to 2.01 mm for all treatments.
Key words: Alkaloids, antibacterial activity, Datura stramonium, gas chromatography-mass specroscopy.


GC-MS, Gas chromatography-mass spectroscopy.