African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12196

Full Length Research Paper

Somatic embryogenesis and plant regeneration in popcorn (Zea mays L.) inbred lines

Ana Luisa Monezi Lucena
  • Ana Luisa Monezi Lucena
  • Postgraduate Program in Genetics and Breeding, State University of Maringá, 87020-900 Maringá, PR, Brazil.
  • Google Scholar
Ivone Batista de Oliveira Eloi
  • Ivone Batista de Oliveira Eloi
  • Postgraduate Program in Genetics and Breeding, State University of Maringá, 87020-900 Maringá, PR, Brazil.
  • Google Scholar
Carlos Alberto Scapim
  • Carlos Alberto Scapim
  • Department of Agronomy, State University of Maringá, 87020-900 Maringá, PR, Brazil.
  • Google Scholar
Claudete Aparecida Mangolin
  • Claudete Aparecida Mangolin
  • Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, 87020-900 Maringá, PR, Brazil.
  • Google Scholar
Maria de Fátima P. S. Machado
  • Maria de Fátima P. S. Machado
  • Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, 87020-900 Maringá, PR, Brazil.
  • Google Scholar


  •  Received: 07 November 2016
  •  Accepted: 23 December 2016
  •  Published: 16 August 2017

Abstract

The objective of this study was to evaluate the production of embryogenic calluses and plant regeneration of seven popcorn inbred lines (Zea mays L.) in order to identify lines for further transference of genes to a generation of transgenic plants. The N6 medium with supplementation was used to induce calluses in immature embryos, and supplemented MS medium was used to stimulate plant regeneration of P11, P12, P15 and P18 inbreed lines. The number of regenerated plants was the highest for the P15 inbred line. The P15 popcorn inbred line represents a potential genotype for transferring plant regeneration competence to other popcorn genotypes.

Key words: Maize, immature embryos, callus induction, somatic embryos, regeneration competence.