African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12400

Full Length Research Paper

Development of a potential lignocellulolytic resource for rapid bioconversion of rice straw

H.  Kausar 1, M. Sariah*1,2, M. Razi Ismail2,3  Halimi Mohd Saud4, S. H. Habib5, and Zulkarami Berahim2
  1Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. 2Laboratory of Food Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. 3Department of Crops Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. 4Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. 5Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Email: [email protected]

  •  Accepted: 10 April 2012
  •  Published: 10 May 2012

Abstract

 

This study was conducted at the Laboratory of Food Crops and Floriculture, Institute of Tropical Agriculture, Universiti Putra Malaysia to characterize the potential lignocellulolytic bacteria for rapid bio-bioconversion of rice straw. Fifty isolates of bacteria were isolated from several in-situ and in-vitro sources. Isolates B3, B13, B21, B37 and B46 showed the highest enzymatic activities to starch, carboxymethyl cellulose (CMC) and Azure B amended media. They were further screened on rice straw powder (RSP) amended media for their adaptability on rice straw (RST). The isolate B37 obtained from thermophilic phase of in vitro composting of rice straw and showing the optimum lignocellulolytic activity and adaptation to RSP amended media was identified as Bacillus pumilis.

 

Key words: Rice straw, lignocellulose, microbial degradation, Bacillus pumilis.