African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

Seed origin, storage conditions, and gibberellic acid on in vitro germination of Campomanesia adamantium (Cambess.) O. Berg

Claudia Roberta Damiani
  • Claudia Roberta Damiani
  • Laboratório de Biotecnologia Vegetal - Faculdade de Ciências Biológicas e Ambientais (FCBA), Universidade Federal da Grande Dourados (UFGD) - Rodovia Dourados - Itahum, Km 12, CEP: 79804-970, Dourados – MS, Brazil.
  • Google Scholar
Leandro Darc da Silva
  • Leandro Darc da Silva
  • Laboratório de Biotecnologia Vegetal - Faculdade de Ciências Biológicas e Ambientais (FCBA), Universidade Federal da Grande Dourados (UFGD) - Rodovia Dourados - Itahum, Km 12, CEP: 79804-970, Dourados – MS, Brazil.
  • Google Scholar
Ademir Goelzer
  • Ademir Goelzer
  • Laboratório de Biotecnologia Vegetal - Faculdade de Ciências Biológicas e Ambientais (FCBA), Universidade Federal da Grande Dourados (UFGD) - Rodovia Dourados - Itahum, Km 12, CEP: 79804-970, Dourados – MS, Brazil.
  • Google Scholar
Thamiris Gatti Deo
  • Thamiris Gatti Deo
  • Laboratório de Biotecnologia Vegetal - Faculdade de Ciências Biológicas e Ambientais (FCBA), Universidade Federal da Grande Dourados (UFGD) - Rodovia Dourados - Itahum, Km 12, CEP: 79804-970, Dourados – MS, Brazil.
  • Google Scholar


  •  Received: 14 February 2016
  •  Accepted: 21 July 2016
  •  Published: 10 August 2016

Abstract

Campomanesia adamantium (Cambess.) O. Berg seeds (guavira) are recalcitrant and rapidly lose viability upon removal from the fruit, making difficult the long-term storage. In vitro germination could be used as an important tool to overcome the issues related to this short viability. It might help seed conservation and species propagation. The purpose of this study was to evaluate in vitro germination of guavira seeds collected from different sites and stored under different conditions. Also, the sowing of these seeds in MS medium supplemented with different concentrations of gibberellic acid (GA3) was evaluated. Seeds from the local garden were treated with: 0, 1.0, 2.0, 3.0, or 4.0 mg L-1 GA3. Seeds from a local farmer’s market were treated with: 0, 2.5, 5.0, 7.5 or 10 mg L-1 GA3. We evaluated the germination rate, the average length of the shoot and main root, and the number of leaves. The C. adamantium seeds were sown  immediately after harvest and treated with GA3, regardless concentration, increased germination rate by at least 10%, whereas 1.0 mg L-1 GA3 resulted in 100% germination. The shoot length increased linearly with increasing concentration of the growth regulator. Different concentrations of GA3 had no effect on the development of the main root and leaves. Seeds acquired from a local farmer’s market showed lower germination rate than those sown immediately after harvesting, and did not differ in the rate of germination under different treatments with GA3. Furthermore, around 25% of those seedlings had abnormal leaf morphology. C. adamantium seeds stored at 4°C and -20°C for 60 days did not germinate successfully, suggesting that seeds under cold storage conditions cannot be used for germplasm purposes.

 

Key words:  Guavira, Cerrado, Myrtaceae, temperature.