African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12275

Full Length Research Paper

Assessment of genetic diversity among cultivated Pearl millet (Pennisetum glaucum, Poaceae) accessions from Benin, West Africa

Kifouli ADEOTI
  • Kifouli ADEOTI
  • Département de Biologie Végétale, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, LAMITA, Bénin
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Gustave DJEDATIN
  • Gustave DJEDATIN
  • Faculté des Sciences et Techniques de Dassa Zoumè, Université Polytechnique d’Abomey, Bénin
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Ebenezer EWEDJE
  • Ebenezer EWEDJE
  • Faculté des Sciences et Techniques de Dassa Zoumè, Université Polytechnique d’Abomey, Bénin
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Thierry BEULE
  • Thierry BEULE
  • CIRAD, UMR DIADE (IRD, UM), Montpellier, France
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Sylvain SANTONI
  • Sylvain SANTONI
  • INRA, UMR AGAP (CIRAD, INRA), Montpellier, France
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Alain RIVAL
  • Alain RIVAL
  • CIRAD, DGDRS, Jakarta, Indonesia
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Estelle JALIGOT
  • Estelle JALIGOT
  • CIRAD, UMR DIADE (IRD, UM), Montpellier, France
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  •  Received: 17 January 2017
  •  Accepted: 21 March 2017
  •  Published: 12 April 2017

Abstract

Simple sequence repeat (SSR) molecular markers were used for genetic diversity analysis and population structure of the cultivated Pearl millet in Benin, West Africa. In order to assess the level of genetic diversity, 14 polymorphic SSR markers were used to screen 114 accessions from different agro-ecological zones in Benin. SSR markers were found to reveal a total of 57 alleles with an average of 4.071 allele per locus. Genetic diversity index varied from 0.099 to 0.633 with an average of 0.405. The average observed heterozygosity was found to reach 0.425. The analysis of molecular variance showed no real differentiation between regions. Only 5% of genetic variation was observed between samples collected from north-eastern and north-western region. A high level of variation (95%) was observed among accessions. Moreover, both principal component analysis (PCA) and the dendrogram obtained from the genetic distance among accessions revealed the absence of any specific structuration of accessions from each region under study. Our results confirmed diversity among cultivated Pearl millet in Benin and such diversity is not clustering according to geographical patterns.

 

Key words: Cereal, simple sequence repeat (SSR) markers, genetic variability.