African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Phylogenetic and molecular evolutionary analyses of gypsy group retrotransposon families in the Egyptian cotton Gossypium barbadense

Abdel Ghany A. Abdel Ghany1 and Essam A. Zaki2*
  1Institute of Efficient Productivity, Zagazig University, El Zagazig, Egypt. 2Genetic Engineering & Biotechnology Research Institute, GEBRI, Research Area, Borg El Arab, Post Code 21934, Alexandria, Egypt.   *Corresponding author; Essam A. Zaki, Current Address: Department of Biological Sciences, 1392 Lilly Hall of Life Sciences, West Lafayette
Email: [email protected]

  •  Accepted: 11 August 2003
  •  Published: 30 September 2003

Abstract

 

Gypsy group retrotransposons in the Egyptian cotton, Gossypium barbadense, was  examined by phylogenetic and molecular evolutionary analyses.  DNA sequences ofgypsy group retrotransposons in two G. barbadense cultivars revealed that these sequences are heterogeneous and represent two distinct families. Sequence variation between these families seems to preserve coding information of the reverse transcriptase domain. The high ratio of synonymous to nonsynonymous changes indicates that the reverse transcriptase domain of these families is evolving under purifying selection. Our phylogenetic analysis revealed that the closest relatives of cotton retroelements are found in other plants gypsy group retrotransposons. Cotton retroelements-encoded transcripts were detected in their related respective young seedlings using RNA slot-blot hybridization, suggesting their transcriptional activity. The wide distribution of gypsygroup retrotransposons and the detection of their encoded transcripts illustrate their active role in the Gossypium genome.  

 

Key words: Evolution, Gossypiumgypsy, retrotransposons, reverse transcriptase, substitution rates, transcription.