African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12218

Full Length Research Paper

Common bean germplasm resistant to races 73 and 2047 of Colletotrichum lindemuthianum

Luana Mieko Darben*
  • Luana Mieko Darben*
  • Departamento de Agronomia, Universidade Estadual de Maringá, Av. Colombo, 5.790, Jardim Universitário, 87020-900 Maringá, Parana, Brazil.
  • Google Scholar
Adriana Gonela
  • Adriana Gonela
  • Departamento de Agronomia, Universidade Estadual de Maringá, Av. Colombo, 5.790, Jardim Universitário, 87020-900 Maringá, Parana, Brazil.
  • Google Scholar
Haroldo Tavares Elias
  • Haroldo Tavares Elias
  • Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina, Santa Catarina, Brazil.
  • Google Scholar
Claudete Rosa da Silva
  • Claudete Rosa da Silva
  • Departamento de Agronomia, Universidade Federal Rural da Amazônia, PA 275, Km 13, Zona Rural Parauapebas, PA, 68.515-000, Pará, Brasil.
  • Google Scholar
Heloisa Helena Pastre
  • Heloisa Helena Pastre
  • Departamento de Agronomia, Universidade Estadual de Maringá, Av. Colombo, 5.790, Jardim Universitário, 87020-900 Maringá, Parana, Brazil.
  • Google Scholar
Maria Celeste Gonçalves-Vidigal
  • Maria Celeste Gonçalves-Vidigal
  • Departamento de Agronomia, Universidade Estadual de Maringá, Av. Colombo, 5.790, Jardim Universitário, 87020-900 Maringá, Parana, Brazil.
  • Google Scholar


  •  Received: 24 October 2016
  •  Accepted: 31 January 2017
  •  Published: 10 May 2017

Abstract

The present work aims to identify common bean accessions resistant to anthracnose by incompatibility reaction evaluations to races 73 and 2047 of C. lindemuthianum, associated with using of SF101072 and SAS13950 molecular markers linked, respectively, to gene Co-10 and allele Co-42. A total of 75 common bean accessions from Common Bean Germplasm Bank (CBGB) of Núcleo de Pesquisa Aplicada à Agricultura (Nupagri) were evaluated. The results showed that 14 accessions were resistant to both races 73 and 2047 of C. lindemuthianum, 36 exhibited resistances only to race 2047, and 25 revealed incompatibility reaction to race 73. Joint analysis between incompatibility phenotypic reactions and the presence of markers allowed identifying 13 resistant accessions to races 73 and 21 to race 2047, indicating the occurrence of Co-10 (renamed Co-34) and Co-42, respectively. A total of 32 accessions were resistant without the presence of markers. Among the 75 common bean accessions evaluated, important sources of resistance to C. lindemuthianum, from Andean and Mesoamerican regions, were identified and have promising potential in breeding programs.

Key words: Phaseolus vulgaris, anthracnose, molecular marker, sequence characterized amplified regions for amplification of specific band (SCAR).