Abstract

The present study investigated the black rice ethanolic extract (BREE) protective effect as natural antioxidants against oxidative stress induced by ethyl alcoholic (EA) in male rats. BREE flavonoids and phenols were determined by high performance liquid chromatography (HPLC). The study was conducted on 40 male Wistar rats weighing 170±2 g, the animals divided into 4 equal groups. The first group was given distilled water (DW) and used as a negative control (NC) group. The second group was administrated EA (6 g/kg bw/day) and used as positive control (PC) group. The other groups of rats were administrated [BREE 125 or 250 mg/kg bw + EA 6 g/kg bw/day]. Blood samples were collected after 10 days. Lipid profile, thiobarbituric acid (TBA), superoxide dismutase (SOD), glutathione (GSH) and F2-isoprostanes (F2-isoPs) were determined. The results indicate that the rats treated with EA 6 g/kg bw/day showed a significant (p≤0.05) increase in the levels of total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), TBA and F2-isoprostanes and significant (p≤0.05) decrease in high-density lipoprotein-cholesterol (HDL-C), SOD and GSH levels. The rise in serum TC, LDL-C, TBA and F2-isoprostanes were significantly attenuated by BREE 125 or 250 mg/kg bw + EA 6 g/kg bw/day. Also, the levels of serum HDL-C, SOD and GSH in BREE 125 or 250 mg/kg bw + EA 6 g/kg bw/day groups showed a significant (p≤0.05) increase as compared to PC group. The current results ascertained the beneficial effects of BREE in controlling oxidative stress induced by ethyl alcohol in male rats.

Key words: Antioxidants, black rice extract, ethanol, oxidative stress, free radicals, F2-isoprostanes, rats.