African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12083

Full Length Research Paper

Bioprospecting of yeasts for amylase production in solid state fermentation and evaluation of the catalytic properties of enzymatic extracts

Ana Paula Aguero de Oliveira
  • Ana Paula Aguero de Oliveira
  • Laboratory of Enzymology and Fermentation Processes, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados (FCBA/UFGD), Dourados - MS, Brazil.
  • Google Scholar
Maria Alice Silvestre
  • Maria Alice Silvestre
  • Laboratory of Enzymology and Fermentation Processes, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados (FCBA/UFGD), Dourados - MS, Brazil.
  • Google Scholar
Heloiza Ferreira Alves-Prado
  • Heloiza Ferreira Alves-Prado
  • Faculty of Engineering, Department of Phytotechnology, Food Technology and Social Economy, Sao Paulo State University (FEI/UNESP), Ilha Solteira - SP, Brazil.
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Andre Rodrigues
  • Andre Rodrigues
  • Laboratory of Fungal Ecology and Systematics, Biosciences Institute, Department of Biochemistry and Microbiology, Sao Paulo State University (IB/UNESP), Rio Claro - SP, Brazil.
  • Google Scholar
Marcelo Fossa da Paz
  • Marcelo Fossa da Paz
  • Laboratory of Enzymology and Fermentation Processes, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados (FCBA/UFGD), Dourados - MS, Brazil.
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Gustavo Graciano Fonseca
  • Gustavo Graciano Fonseca
  • Laboratory of Bioengineering, Faculty of Engineering, Federal University of Grande Dourados (FAEN/UFGD), Dourados - MS, Brazil.
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Rodrigo Simoes Ribeiro Leite*
  • Rodrigo Simoes Ribeiro Leite*
  • Laboratory of Enzymology and Fermentation Processes, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados (FCBA/UFGD), Dourados - MS, Brazil.
  • Google Scholar


  •  Received: 21 July 2014
  •  Accepted: 17 March 2015
  •  Published: 08 April 2015

Abstract

Profiling microorganisms with potential for amylase production in low cost culture media has been widely recognized due to its broad applicability. The aim of this study was to select yeast strains with potential to produce amylolytic enzymes by solid state fermentation. Fifty-four (54) strains were assessed and three exhibited ability to produce amylases: Candida parapsilosis with 14.68 U/mL (146.8 U/g substrate); Rhodotorula mucilaginosa with 25.0 U/mL (250 U/g substrate), and Candida glabrata with 25.39 U/mL (253.9 U/g substrate), in solid state fermentation, for 120 h at 28°C, using wheat bran with 70% moisture. The enzymes exhibited maximum activity at a pH of 7.0 and at 60°C. Amylases demonstrated satisfactory structural stability, maintaining their catalytic activity after 1 h at 50°C. All enzymes were ethanol tolerant and retained more than 70% of their original activities in 15% ethanol solution. Corn starch was efficiently hydrolyzed by enzymes and the extracts produced by C. parapsilosis and C. glabrata exhibited dextrinizing activity, while those produced by R. mucilaginosa exhibited saccharifying activity.

 

Key words: Candida parapsilosis, Candida glabrata, Rhodotorula mucilaginosa, dextrinizing and saccharifying activity.