African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12182

Full Length Research Paper

Plant cell suspension culture of Thevetia peruviana (Pers.) K. Schum. in shake flask and stirred tank reactor scale: A comparative study

Juan Pablo Arias
  • Juan Pablo Arias
  • Grupo de Biotecnología Industrial, Facultad de Ciencias, Universidad Nacional de Colombia Sede Medellín, Calle 59ª, No 63-20 Bloque 19A-313, Medellín, Colombia.
  • Google Scholar
Karol Zapata
  • Karol Zapata
  • Grupo de Química de los Productos Naturales y los Alimentos, Facultad de Ciencias, Universidad Nacional de Colombia Sede Medellín, Calle 59ª, No 63-20 Bloque 19A-211, Medellín Colombia.
  • Google Scholar
Benjamín Rojano
  • Benjamín Rojano
  • Grupo de Química de los Productos Naturales y los Alimentos, Facultad de Ciencias, Universidad Nacional de Colombia Sede Medellín, Calle 59ª, No 63-20 Bloque 19A-211, Medellín Colombia.
  • Google Scholar
Mariana Penuela
  • Mariana Penuela
  • Grupo de Bioprocesos, Facultad de Ingeniería, Universidad de Antioquia, Calle 70 No. 52-21, Bloque 18-405 Medellín, Colombia.
  • Google Scholar
Mario Arias
  • Mario Arias
  • Grupo de Biotecnología Industrial, Facultad de Ciencias, Universidad Nacional de Colombia Sede Medellín, Calle 59ª, No 63-20 Bloque 19A-313, Medellín, Colombia.
  • Google Scholar


  •  Received: 17 October 2017
  •  Accepted: 22 December 2017
  •  Published: 27 December 2017

Abstract

The aim of this work was to evaluate the effect of changing the shake flask for a stirred-tank bench bioreactor on the performance of Thevetia peruviana cultures. Maximum cell growth of 18.59 and 8.46 g DW/L for shake flask and bench bioreactor at day 14 was obtained, respectively. The end of the exponential growth phase in shake flask was correlated with total sugar consumption, while this behavior was not observed in bench bioreactor. Preferential uptake of glucose over fructose was observed in both systems. Cell morphology was similar for both systems during culture time, exhibiting individual cells with cylindrical and elongated shape at day 0 and some aggregates with rounded cells at day 14. Polyphenol production was not affected by the system configuration and scale. Intra and extracellular antioxidant activity were directly related with phenol production in both systems. Guaiacol peroxidase activity (GPX) went from 1.87 to 7.97 µM/min/mg protein in shake flask and it correlated with low reactive oxygen species (ROS) production. This is an indication of a positive enzymatic cell response for culture in shake flask mediated by GPX. In contrast, the conditions in the bench bioreactor generated a higher stress environment, increasing ROS production without activating enzymatic or non-enzymatic responses led by guaiacol and phenolic compounds, respectively. In conclusion, bench stirred-tank bioreactor scale affects biomass production and sugars uptake negatively. 
 
Key words: Thevetia peruviana, plant cell suspension culture, stirred-tank bioreactor, phenolic compounds, antioxidant activity.

Abbreviation

ABTS, 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid); GAE, gallic acid equivalent; DCFDA, 2’,7’ dichlorofluorescein diacetate; ROS, reactive oxygen species; GPX, guaiacol peroxidase activity; PVP, polyvinylpyrrolidone; TG, tetraguaiacol.