African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12505

Full Length Research Paper

Isolation and selection of Bradyrhizobium from the root nodules of indigo plants (Indigofera tinctoria L.)

A. K. M. Mosharof Hossain1, Musharraf Hossain Mian2, M. A. Hakim3, M. M. Islam4* and Jannatul Ferdous3
1Department of Soil Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh. 2Depertment of Soil Science, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh. 3Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. 4Department of Forest Management, Faculty of Forestry, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Email: [email protected]

  •  Accepted: 13 June 2012
  •  Published: 31 July 2012

Abstract

This research was conducted from 2004 to 2005 to isolate and select Bradyrhizobiumfrom the root nodules of indigo plants (Indigofera tinctoria L.). For isolation ofBradyrhizobium, root nodules were collected from indigo plants. FourteenBradyrhizobium isolates were identified depending on colony, morphological and biochemical characteristics. Out of fourteen isolates, six (HSTU-IR2, HSTU-IR3, HSTU-IR4, HSTU-IR9, HSTU-IR10 and HSTU-IR14) were found promising with respect to nodulation, shoots and roots weights and N fixation by the indigo plants grown inside the Leonard Bottle Jar Assembly (LBJA).

 

Key words: Bradyrhizobium, isolation, selection, root nodules, indigo plants.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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