African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12193

Full Length Research Paper

In silico mining of micro-RNAs from Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

R. Asokan
  • R. Asokan
  • Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Karnataka, Bengaluru, 560 089, India
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H. K. Roopa
  • H. K. Roopa
  • Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Karnataka, Bengaluru, 560 089, India
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K. B. Rebijith
  • K. B. Rebijith
  • Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Karnataka, Bengaluru, 560 089, India
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H. H. Ranjitha
  • H. H. Ranjitha
  • Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Karnataka, Bengaluru, 560 089, India
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N. K. Krishna Kumar
  • N. K. Krishna Kumar
  • Division of Horticulture, ICAR, Krishi Anusandhan Bhawan - II, New Delhi - 110 012, India
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  •  Received: 09 October 2013
  •  Accepted: 17 December 2013
  •  Published: 01 January 2014

Abstract

MicroRNAs (miRNAs) are small, endogenously, non-coding genes that regulate protein production either by mRNA cleavage or by translational repression in eukaryotes and viruses. miRNAs plays a key role in biological processes including growth, development and physiology of an organism. In this study, we employed insilico approaches to identify the miRNAs from Spodoptera frugiperda, a major pest of small grain crops. A total of seven miRNAs were identified and characterized from 67,360 expressed sequence tags (ESTs) of S. frugiperda with: 1) mature and pre-miRNAs sizes vary from 19 to 25 ans 61 to 95 nucleotides respectively; 2) minimum free energy ranged from -31.70 to -21.00 kcal/mol; and 3) (A + U) content varied from 27 to 60. The functional annotation of these miRNAs were identified as regulation of transcription factors, catalytic activities and signal transduction pathways. Further studies of these miRNAs will help to carryout functional analyses, which promises more towards insect pest management  free of insecticides and pesticides.

 

Key words: MicroRNAs, translational repression, Spodoptera frugiperda, expressed sequence tags, minimum free energy, insect pest management.