African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Dihydrotestostenone increase the gene expression of androgen receptor coregulator FHL2 in human non-transformed epithelial prostatic cells

Adriane Pozzobon*
  • Adriane Pozzobon*
  • Centro de Ciencias Biologicas e da Saude, Univates, Lajeado, Rio Grande do Sul, Brazil.
  • Google Scholar
Diego B Pianta
  • Diego B Pianta
  • Laboratorio de Biologia Endocrina e Tumoral, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
  • Google Scholar
Maria Flávia M Ribeiro
  • Maria Flávia M Ribeiro
  • Laboratorio de Biologia Endocrina e Tumoral, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
  • Google Scholar
Ilma Simoni Brum
  • Ilma Simoni Brum
  • Laboratorio de Biologia Endocrina e Tumoral, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
  • Google Scholar


  •  Received: 16 February 2015
  •  Accepted: 01 June 2015
  •  Published: 03 June 2015

Abstract

The actions of androgens are mediated through an androgen receptor (AR), and AR activity is modulated by coregulators. The aim of this study was to assess the action of androgens in the expression of AR and the coregulators FHL-2 and SHP-1 in human non-transformed epithelial prostatic cells (HNTEP) treated with androgens. Prostate tissues were obtained from 12 patients between 60 and 77 years of age. HNTEP cells were grown in basal medium and treated with DHT in different conditions. HNTEP cells under treatment with DHT (10-13 M) induced an increase in FHL-2 expression. In turn, high DHT concentrations (10-8 M) induced an increase in the expression SHP-1. The present data suggest that the SHP-1 and FHL-2 genes play a role in the control of responsiveness and androgen-dose-dependent cell proliferation in HNTEP cells. Further studies are needed to assess the influence of androgens in AR and its coregulators and the implications in the pathophysiology of prostate diseases.
 
Key words: Androgens, FHL-2, AR, prostate, proliferation, coregulators.
 

Abbreviation

AR, Androgen receptor; HNTEP, human non-transformed epithelial prostatic cells; LBD, ligand binding domain; DBD, DNA-binding domain; NTD, NH2- terminal transactivation domain; AF-1, activation function domain 1; BPH, benign prostate hyperplasia; PCa, prostate carcinoma; LUTS, lower urinary tract symptoms; ARE, androgen responsive elements; SDS, sodium dodecyl sulfate; Tm, melting temperature; CT, cycle threshold; SEM, standard error of mean; SPSS, statistical package for social sciences.