African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12392

Full Length Research Paper

Metabolic engineering of Neurospora crassa for increasing carotenoids synthesis

Sylvia E. Brown
  • Sylvia E. Brown
  • College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
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Chun Lin
  • Chun Lin
  • College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
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Zhengjie Liu
  • Zhengjie Liu
  • College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
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Hao Wen
  • Hao Wen
  • College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
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Qin Cheng
  • Qin Cheng
  • College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
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Zichao Mao
  • Zichao Mao
  • College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
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  •  Received: 12 December 2021
  •  Accepted: 14 March 2022
  •  Published: 30 April 2022

Abstract

Carotenoids are essential nutrient compounds with numerous biological functions. Neurospora crassa is a model filamentous fungus with orange pigmentation which is attributed to the accumulation of carotenoids containing neurosporaxanthin (NX) and neutral carotenoids (NC). To enhance carotenoids synthesis in N. crassa, isoprene diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) were increased using the genes, xylulose-5-phosphate phosphoketolase (XPK), phosphotransacetylase (PTA), and NADH-specific-3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMGR), as single, fused and three combined expressions to channel more carbon source into the mevalonate pathway (MVP). The single (PTA, XPK, HMGR), fused (PTA:HMGR & XPK:HMGR) and three combined gene (PTA with fused XPK:HMGR) expressions in engineered fungal resulted in carotenoid titers with contents of NX accumulated up to 4.5 mg/g DW and NC up to 1.7 mg/g DW as compared to the wild-type with NX up to 1.54 mg/g DW and NC up to 0.8 mg/g DW. The optimized MVP with metabolic engineering methods is a key method to increase the synthesis of carotenoid and other active terpenoids in N. crassa.

 

Key words: Neurospora crassa, mevalonate pathway, carotenoids, neurosporaxanthin, neutral carotenoids.