African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12275

Short Communication

Protocol optimization for in vitro shoot multiplication of Jackfruit (Artocarpus heterophyllus L.)

Jemal Ali
  • Jemal Ali
  • Department of Biotechnology, University of Gondar, Gondar, Ethiopia.
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Kassahun Bantte
  • Kassahun Bantte
  • Department of Plant Sciences and Horticulture, Jimma University, Jimma Ethiopia.
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Tileye Feyissa
  • Tileye Feyissa
  • Institute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia.
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  •  Received: 23 December 2015
  •  Accepted: 09 May 2016
  •  Published: 11 January 2017

Abstract

Jackfruit (Artocarpus heterophyllus L.) is a cross pollinated fruit tree valued mainly for its fruit and timber wood. Although the crop has been introduced to Ethiopia and adapted well to the Jimma area, its potential for production has not been exploited due to the absence of an efficient method for large scale propagation. Micropropagation is a method for production of large number of genetically uniform planting materials with desired characteristics. The present study was initiated to determine the optimum concentration and combination of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) for in vitro shoot multiplication. For this study, shoot tips were used as an explant source. The shoot tips from mature trees were sterilized with 70% ethanol and HgCl2 before culturing on Murashige and Skoog (MS) medium supplemented with different combinations of BAP and NAA. The results show that, the combination of BAP and NAA resulted in significant (P<0.01) differences for shoot number, shoot length and leaf number, whereby 2 mg/L BAP alone was found to be the best with a mean shoot number of 5.12 and length of 0.89 cm. The optimized protocol could be used as a baseline for further studies on in vitro propagation of jackfruit.

 

Key words: Artocarpus heterophyllus L., mercuric chloride, auxiliary shoot.