African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12209

Full Length Research Paper

Molecular characterization, polymorphism of growth differentiation factor 5 gene and association with ultrasound measurement traits in native Chinese cattle breeds

Yongfeng Liu1#, Yang Jiao1#, Linsen Zan2*, Yaping Xin1, Linqiang Li1 and Wanqiang Tian1
1College of Animal Science and Technology, Northwest A and F University, Yangling, Shaanxi 712100, PR China. 2National Beef cattle improvement centre of China, Yangling, Shaanxi 7 12100, PR China.
Email: [email protected]

  •  Accepted: 18 June 2010
  •  Published: 31 August 2010

Abstract

Growth differentiation factor 5 (GDF5), involved in the development and maintenance of bone and cartilage, is an important candidate gene for growth and carcass traits selection through marker-assisted selection (MAS). Genomic structural analysis showed that bovine GDF5 shares much similarity with humanGDF5. The latest findings demonstrate that the single nucleotide polymorphism (SNP) T586C in exon 1 is significantly associated with ultrasound marbling score (UMAR) and ultrasound backfat thickness (UBF). Furthermore, the analysis of T586C SNP marker shows there are significant effects on the UBF (P = 0.0498) and on the UMAR (P = 0.0058) in 465 individuals. These results clearly suggest that the GDF5 gene is among target genes for carcass traits in bovine reproduction and breeding.

 

Key words: Cattle, GDF5 gene, ultrasound measurement, polymorphism, association analysis.