African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12508

Full Length Research Paper

Analysis of diversity genetic of Moroccan net blotch populations using amplified fragment length polymorphism (AFLP) markers

Bentata, F.1*, Labhilili, M.4, El Aissami, A.3, Bary, S.4, Yeslem, C. H.4 and Ibijbijen, J.2
1Breeding, Conservation and Valorisation of Phytogenetic Resources Unit, Laboratory of Phytopathology, CRRAR, INRA, B.P 6356, Avenue Mohamed Belarbi Alaoui Rabat, Morocco. 2Environment and Soil Microbiology Unit, Faculty of Sciences, Moulay Ismail University, B.P. 11201 Zitoune, Meknes, Morocco. 3Laboratory of Botanique, Mycology and Environment, Département Biologie,Faculty of Sciences, Mohamed V University, B.P. 1014. Avenue Ibn Battouta B, Rabat, Morocco. 4Biotechnology Unit, Laboratory of Molecular Biology, CRRAR, INRA, B.P 6356, Avenue Mohamed Belarbi Alaoui Rabat, Morocco.
Email: [email protected]

  •  Accepted: 15 June 2011
  •  Published: 27 July 2011

Abstract

Net blotch caused by Pyrenophora teres f. teres is the most harmful foliar disease in barley generating significant economic losses in Morocco. Populations of P. teres f. teres were collected from different regions of Morocco. Thirty five (35) P. teres f. teres isolates, single conidial, were isolated and were subjected to molecular study using amplified fragment length polymorphism (AFLP) technique. Out of thefourteen primers combinations tested, four primers combinations were selected to disclose the polymorphism between the different P. teres f. teres isolates. The molecular characterization of these isolates showed high degree of polymorphism reaching 95% and identifying 25 specific genotypes. The genetic variability of the different isolates of P. teres f. teres within and between Moroccan regions was highlighted, disclosing no linkage between the isolates and their geographical origins. This result might be due to informal material flow between regions.

 

Key words: Barley, net blotch, Pyrenophora teres f. teres, amplified fragment length polymorphism (AFLP), genetic diversity.

Abbreviation

PTT, Pyrenophora teres f. teres; PT, Pyrenophora teres; GS,genetic similarity; dd, double distilled.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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