African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12433

Full Length Research Paper

The effects of different indole-3-butyric acid (IBA) concentrations, two light regimes of in vitro rooting and acclimatization of in vitro teak (Tectona grandis L.f) plantlets

Nor Aini, A. S.1,2*, Goh, B. L.2 and Ridzuan, R.1
  1Laboratory of Sustainable of Bioresources Management, Institute of Tropical Forestry and Forests Products (INTROP), Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. 2Department of Forest Production, Faculty of Forestry, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Email: [email protected]

  •  Accepted: 14 June 2009
  •  Published: 16 November 2009



Effects of different indole-3-butyric (IBA) concentrations (0, 0.5, 1.0, 2.0, 3.0 and 5.0 mg/l), two light regimes of in vitro rooting and acclimatization on in vitro teak (Tectona grandis L.f) were investigated. Shoots incubated in the light produced higher mean number of roots (2.0) and mean root length of 15.0 and 4.5 mm when supplemented with low IBA concentrations of 0.5 and 1.0 mg/l, respectively. Conversely, when shoots were incubated in the dark, higher mean number of roots (8.0 and 3.0) and mean root lengths (14.0 and 8.8 mm) were produced when supplemented with higher IBA concentrations of 2.0 and 3.0 mg/l, respectively. IBA was found necessary for root induction and growth in light and dark where 2.0 mg/l was optimum as it recorded high mean number of root (6.0 and 8.0) with mean root lengths of 11.8 and 14.0 mm, respectively. High survival percentage of 80% was recorded for those plantlets raised in “jiffy-7” but survival reduced to 40% when grown in the autoclaved soil after one week.   


Key words: Teak (Tectona grandis) plantlets, indole-3-butyric acid, light regime, in vitro rooting, acclimatization.