African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Effect of arbuscular mycorrhizal fungal inoculation on growth, and nutrient uptake of the two grass species, Leptochloa fusca (L.) Stapf and Sporobolus robustus Kunth, under greenhouse conditions

Fatoumata Fall*
  • Fatoumata Fall*
  • Departement de Biologie Vegetale, Universite Cheikh Anta Diop, BP 5005 Dakar, Senegal ; Laboratoire Commun de Microbiologie (LCM) IRD/ISRA/UCAD, Centre de Recherche de Bel-Air, Route des Hydrocarbures, BP 1386 Dakar, Senegal; Laboratoire Mixte International Adaptation des Plantes et microorganismes associes aux Stress Environnementaux (LAPSE), BP 1386 Dakar, Senegal.
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Diégane Diouf
  • Diégane Diouf
  • Departement de Biologie Vegetale, Universite Cheikh Anta Diop, BP 5005 Dakar, Senegal ; Laboratoire Commun de Microbiologie (LCM) IRD/ISRA/UCAD, Centre de Recherche de Bel-Air, Route des Hydrocarbures, BP 1386 Dakar, Senegal; Laboratoire Mixte International Adaptation des Plantes et microorganismes associes aux Stress Environnementaux (LAPSE), BP 1386 Dakar, Senegal.
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Dioumacor Fall
  • Dioumacor Fall
  • Laboratoire Commun de Microbiologie (LCM) IRD/ISRA/UCAD, Centre de Recherche de Bel-Air, Route des Hydrocarbures, BP 1386 Dakar, Senegal; Laboratoire Mixte International Adaptation des Plantes et microorganismes associes aux Stress Environnementaux (LAPSE), BP 1386 Dakar, Senegal; Universite des Antilles et de la Guyane97159, Pointe-a-Pitre, Guadeloupe, France.
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Ibrahima Ndoye
  • Ibrahima Ndoye
  • Departement de Biologie Vegetale, Universite Cheikh Anta Diop, BP 5005 Dakar, Senegal ; Laboratoire Commun de Microbiologie (LCM) IRD/ISRA/UCAD, Centre de Recherche de Bel-Air, Route des Hydrocarbures, BP 1386 Dakar, Senegal; Laboratoire Mixte International Adaptation des Plantes et microorganismes associes aux Stress Environnementaux (LAPSE), BP 1386 Dakar, Senegal.
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Cheikh Ndiaye
  • Cheikh Ndiaye
  • Laboratoire Commun de Microbiologie (LCM) IRD/ISRA/UCAD, Centre de Recherche de Bel-Air, Route des Hydrocarbures, BP 1386 Dakar, Senegal; Laboratoire Mixte International Adaptation des Plantes et microorganismes associes aux Stress Environnementaux (LAPSE), BP 1386 Dakar, Senegal.
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Aboubacry Kane
  • Aboubacry Kane
  • Departement de Biologie Vegetale, Universite Cheikh Anta Diop, BP 5005 Dakar, Senegal ; Laboratoire Commun de Microbiologie (LCM) IRD/ISRA/UCAD, Centre de Recherche de Bel-Air, Route des Hydrocarbures, BP 1386 Dakar, Senegal; Laboratoire Mixte International Adaptation des Plantes et microorganismes associes aux Stress Environnementaux (LAPSE), BP 1386 Dakar, Senegal.
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Amadou Mustapha Ba
  • Amadou Mustapha Ba
  • Laboratoire de Biologie et Physiologie Vegetales, Universite des Antilles, 97159, Pointe-a-Pitre, Guadeloupe, France; Laboratoire des Symbioses Tropicales et Méditerranéennes, UMR113 INRA/AGRO-M/CIRAD/IRD/UM2 - TA10/J, Campus International de Baillarguet, 34398 Montpellier cedex 5, France.
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  •  Received: 06 June 2015
  •  Accepted: 14 September 2015
  •  Published: 30 September 2015

Abstract

The aim of our work was to assess the effect of inoculation with three arbuscular mycorrhizal fungi (AMF) (Rhizoglomus aggregatum (N.C. Schenck and G.S. Sm.) Sieverd., G.A. Silva and Oeh., Funneliformis mosseae (T.H. Nicolson and Gerd.) C. Walker and A. Schüssler. and Rhizoglomus intraradices (N.C. Schenck and G.S. Sm.) Sieverd., G.A. Silva and Oehl.), and a mixed inoculum of these AMF on root colonization, biomass production, mycorrhizal dependency (MD) and shoot mineral contents of two salt tolerant grasses Leptochloa fusca L. Stapf and Sporobolus robusts Kunth. After four months of growth in a sterilized soil and greenhouse conditions, grasses inoculated with AMF showed significantly higher total biomass production than non-inoculated seedlings. MD and shoot mineral contents (especially P) varied with AMF host plants. Maximum values of MD (13%) were observed in L. fusca and S. robustus seedlings when inoculated with R. intraradices and F. mosseae, respectively. Only P contents were higher in the S. robustus/mixed-AMF combinations than the other treatments. These results demonstrate the potential benefits in our experimental conditions of AM inoculation for improving growth and P acquisition particularly in the L. fusca/ F. mosseae and S. robustus/mixed-AMF combinations.

Key words: Grass species, symbiosis, mycorrhizal dependency, mineral nutrition.

Abbreviation

AMF, Arbuscular mycorrhizal fungi; MD, mycorrhizal dependency.