Abstract

The objective of this work was the optimization of the conditions of callus and cell suspension culture of Elaeagnus angustifolia for the production of condensed tannins. The effects of different conditions on the callus growth and the production of condensed tannins were researched. The leaf tissue part of E. angustifolia was optimum explant of callus induction. The best callus growth and the highest condensed tannins production was obtained under the culture on MS basal medium containing 1.00 mg·L^-1 2,4-D and 0.50 mg·L^-1 BA. The optimum time of subculture was 20 - 25 d. The results showed the contents of condensed tannins decreased gradually at the beginning of the callus differentiation. Vigorous and friable callus was used for cell suspension culture. Study on the effect of medium and thehormone combination showed that plenty of incompact and rapid growing suspension cells were obtained from cultures grown in liquid medium supplemented with 0.1 mg·L^-1 BA + 0.1 mg·L^-1 TDZ. The study provided an efficient way for E. angustifolia cell suspension culture to produce secondary metabolite.

Key words: Elaeagnus angustifolia L, callus, condensed tannins, suspension culture.

Abbreviation

Abbreviations: PGR, plant growth regulator; DW, dry weight; FW, fresh weight; 2,4-D, 2,4-dichlorophenoxyacetic acid; BA, 6-bezyladenine; NAA, α-naphthaleneacetic acid; IAA, Indole-3-acetic acid.