African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12427

Full Length Research Paper

High genetic diversity of Mycospaherella graminicola (Zymoseptoria tritici) from a single wheat field in Tunisia as revealed by SSR markers

Samia Berraies1, Mohamed Salah Gharbi2, François Belzile3, Amor Yahyaoui4, Mohamed Rebah Hajlaoui 5, Mokhtar Trifi 1, Martine Jean3 and Salah Rezgui6
1Laboratoire de génétique moléculaire, immunologie et biotechnologie Faculté des Sciences de Tunis, Tunisie. 2Laboratoire de grande culture et de protection des végétaux, Institut National de Recherche Agronomique de Tunis, Tunisie. 3Département de Phytologie, Pavillon Charles-Eugene-Marchand, Faculté des sciences de l’agriculture et de l’alimentation, Université Laval, Québec, Canada. 4International Maize and Wheat Improvement Center (CIMMYT) Mexico, Mexico 5Laboratoire de Biotechnologie Agricole et Alimentaire. Institut National de Recherche Agronomique de Tunis, Rue Hédi Karray, 2049, Tunis Tunisie. 6Laboratoire de Génétique et d’Amélioration des Plantes, Institut National Agronomique de Tunis, Tunisie.
Email: [email protected]

  •  Published: 31 March 2013


Microsatellite markers were used to assess genetic diversity of Mycosphaerella graminicola at a micro geographical scale in Tunisia. Forty five (45) isolates were sampled and assessed using seven pairs of single-locus microsatellite primers not previously tested on populations of M. graminicola in Tunisia. Genetic diversity of the field population ranged from 0.403 to 0.555 with an average of 0.484. A high level of genetic diversity was found at a sharp scale throughout the pathogen population tested. Among 45 isolates sampled, 39 different multi locus genotypes (MLG) were identified. Cluster analysis (UPGMA) showed that 86% of the isolates tested were distinct. The high degree of DNA polymorphism, the large number of different molecular genotypes and the pattern of cluster analysis suggest that sexual ascospores and/or asexual spores of a highly mutable local population could have contributed to the genetic diversity of M. graminicola in Tunisia.


Key words: Durum wheat, genetic diversity, microsatellites, Mycosphaerella graminicola, micro geographical scale, Tunisia.