African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12421

Full Length Research Paper

Acute and sub-chronic toxicity studies of methanolic extract of Tulbaghia violacea rhizomes in Wistar rats

  O. S. Olorunnisola1, G. Bradley1 and A. J. Afolayan2*  
  1Department of Biochemistry and Microbiology, University of Fort Hare, Private   Bag   X1314, Alice 5700, South Africa. 2Phytomedicine Research Group, Department of Botany, University of Fort Hare, Alice, 5700, South Africa.
Email: [email protected]

  •  Accepted: 26 June 2012
  •  Published: 16 October 2012

Abstract

 

The methanolic extract of the rhizome of Tulbaghia violacea (RTV) was tested for toxicity in albino Wistar rats. A single oral administration of a 5 g/kg dosage of RTV extract did not produce mortality or significant behavioral changes during 14 days of observation. In the sub-chronic study, the extract administered daily for a period of 28 days showed no mortality or morbidity and the relative weights of organs were not affected by the treatment. However, significant weight gain in both treated and control group was observed. Indicators of liver damage such as alanine amino transferase (ALT) and aspartate amino transferase (AST), as well as total serum protein and albumin showed no significant alteration in the extract treated animals when compared with the controls at all doses used. The extracts caused a significant (p<0.05) decrease in the activity of gamma glutamy transferase (Æ”GT) and alkaline phosphatase (ALP) when compared with the control. However, the extract did not have any significant effect on markers of kidney function or hematological parameters in the rats, except in the female rats where a significant increase (p<0.05) in lymphocytes counts at 500 mg/kg was observed. Histopathological examination of the liver revealed no detectable inflammation. These results suggest that the rhizomes ofT. violacea may be potentially safe for consumption.

 

Key words: Tulbaghia violacea, rhizomes, acute toxicity, subchronic toxicity.