African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12196

Full Length Research Paper

Antibacterial and antioxidant activity of silver nanoparticles synthesized using aqueous extract of Moringa stenopetala leaves

Abambagade Abera Mitiku
  • Abambagade Abera Mitiku
  • Department of Chemistry, Arba Minch College of Teachers Education, Southern Nations, Nationalities and Peoples' Region, Ethiopia.
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Belete Yilma
  • Belete Yilma
  • Department of Chemistry, Arba Minch University, College of Natural Sciences, Southern Nations, Nationalities and Peoples' Region, Ethiopia.
  • Google Scholar


  •  Received: 29 March 2017
  •  Accepted: 31 July 2017
  •  Published: 09 August 2017

Abstract

Green synthesis of silver nanoparticles (AgNPs) is non-toxic and eco-friendly than commonly used physicochemical methods. The study focuses on synthesis, characterization, antibacterial and antioxidant activity of AgNPs synthesized using aqueous extract of Moringa stenopetala (M. stenopetala) leaves. Optimum heating time required for the preparation of aqueous extract of M. stenopetala leaves was 15 min. It was found that 90 ml of 1 mM of silver nitrate solution and 15 ml of aqueous extract of M. stenopetala leaves were more favorable for maximum production of AgNPs with a yield of 97.14%. Ultraviolet-visible (UV-Vis) spectrum of synthesized AgNPs shows a peak at 412 nm. Fourier transform infrared spectroscopy (FTIR) spectral analysis indicated involvement of -C=O, -O-H, and -N-H in the formation of AgNPs. X-ray diffraction (XRD) pattern showed the face centered cubic (FCC) structure of AgNPs with average particle size of 11.44 nm. Synthesized AgNPs showed stronger antibacterial activity against Escherichia coli than Staphylococcus aureus and has better antioxidant activity than standard ascorbic acid.

Key words: Silver nanoparticles (AgNPs), green synthesis, Moringa stenopetala.