African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

High temperature effects on flavones accumulation and antioxidant system in Scutellaria baicalensis Georgi cells

Yuan Yuan1#, Yunjun Liu2#, Yujian Luo1, Luqi Huang1*, Shunqin Chen1, Zhaochun Yang1 and Shuangshuang Qin1          
1Institute of Chinese Material Medical, Academy of Chinese Medical Sciences, Beijing 100700, China. 2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Email: [email protected]

  •  Accepted: 06 April 2011
  •  Published: 13 June 2011

Abstract

Scutellaria baicalensis is a traditional Chinese medicinal plant that has long been grown in Hubei Province. However, increasing average annual temperatures in the region have made plants unsuitable for medicinal use. Two flavones, baicalin and baicalein, are the major active ingredients of S. baicalensis. We demonstrated that protracted heat treatment inhibited the accumulation of baicalin and baicalein as well as the activity of phenylalanine ammonla-lyase (PAL). PAL is involved in the phenylpropanoid pathway, which produces baicalin in the plant. Heat treatment also affected the activities of the antioxidant enzymes such as catalase (CAT), superoxide dimutase (SOD) and peroxidase (POD). However, superoxide radical and hydrogen peroxide content of the cells were not significantly impacted by heat treatment. Cells continued growing during the protracted heat stress. Hence, the increasing temperatures in Hubei Province will very likely make S. baicalensis unsuitable for medical use by decreasing the flavonoid content.

 

Key words: Scutellaria baicalensis Georgi, heat, antioxidant system, flavonoid.