African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12359

Full Length Research Paper

Isolation and characterization of a novel keratinolytic bacterial strain from soil samples of chicken feather keratinaceous-waste dumping sites of Ngaoundere (Cameroon)

Sileu Dombou Armelle Leslie
  • Sileu Dombou Armelle Leslie
  • Department of Process Engineering, National School of Agro-Industrial Sciences, University of Ngaoundere, P. O. Box 455, Ngaoundere, Cameroon.
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Adjia Zangue Henriette
  • Adjia Zangue Henriette
  • Department of Industrial Chemistry and Environment, National School of Agro-Industrial Sciences, University of Ngaoundere, P. O. Box 455, Ngaoundere, Cameroon.
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Nso Emmanuel Jong
  • Nso Emmanuel Jong
  • Department of Process Engineering, National School of Agro-Industrial Sciences, University of Ngaoundere, P. O. Box 455, Ngaoundere, Cameroon.
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Njintang Yanou Nicolas
  • Njintang Yanou Nicolas
  • Department of Biology, Faculty of Sciences, University of Ngaoundere, P. O. Box 455 Ngaoundere, Cameroon.
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Tatsadjieu Ngoune Leopold
  • Tatsadjieu Ngoune Leopold
  • Department of Food Engineering and Quality Control, University Institute of Technology, University of Ngaoundere, P. O. Box 454 Ngaoundere, Cameroon.
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  •  Received: 13 August 2019
  •  Accepted: 13 December 2019
  •  Published: 30 April 2021

Abstract

This study aims at characterizing new chicken feather-degrading bacteria isolated from soils of keratinic waste collected from four dumping sites of Ngaoundere. Fifteen proteolytic bacteria were isolated with three (D1, D2 and F1) showing the capacity to degrade feather in Basal Salt Media. Highest degradation rate (71.11 ± 3.05%) of feather was obtained from isolate D2 from Municipal slaughterhouse. The partial characterization of the strain D2 based on morphological and biochemical tests revealed that it is a Bacillus sp. The keratinolytic activity was positively correlated to the bacterial growth with the highest value (13.76 U.ml-1) obtained at the stationary phase after 120 h of incubation. The optimal conditions for the keratinolytic activity of this isolate were determined to be pH 8.0 and temperature of 45°C. Thus, the Bacillus strain (D2) isolated is a promising strain for the management and valorization of feather wastes.

 

Key words: Chicken feather-degrading bacteria, keratinolytic enzyme activity, characterization, Ngaoundere, Cameroon.