African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Detection of Pseudomonas fluorescens from broth, water and infected tissues by loop-mediated isothermal amplification (LAMP) method

Pooja Saharan,
  • Pooja Saharan,
  • Department of Biotechnology, Chaudhary Devi Lal University, Sirsa-125 055, India.
  • Google Scholar
Joginder S. Duhan
  • Joginder S. Duhan
  • Department of Biotechnology, Chaudhary Devi Lal University, Sirsa-125 055, India.
  • Google Scholar
Suresh K. Gahlawat*
  • Suresh K. Gahlawat*
  • Department of Biotechnology, Chaudhary Devi Lal University, Sirsa-125 055, India.
  • Google Scholar


  •  Received: 10 December 2014
  •  Accepted: 25 March 2015
  •  Published: 08 April 2015

Abstract

Loop mediated isothermal amplification is rapid, highly sensitive and specifically developed method for detection of bacterial infections. AprX gene for alkaline metalloprotease of Pseudomonas fluorescens was used to design four primers and loop mediated isothermal amplification (LAMP) conditions were standardized for amplification of DNA. LAMP primers successfully amplified P. fluorescens from DNA and bacterial cells taken directly from broth, water and infected tissues with high specificity and sensitivity (10 pg) under isothermal condition at 61°C.

 

Key words: Pseudomonas fluorescens, loop mediated isothermal amplification (LAMP), rapid, simple, specificity, sensitivity.