African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Optimization of cultural conditions for protease production by Bacillus subtilis EFRL 01

A.Sattar Qureshi*, M. Aqeel Bhutto, Imrana Khushk and M.Umar Dahot        
Institute of Biotechnology and Genetic Engineering, University of Sindh Jamshoro, Pakistan.
Email: [email protected]

  •  Accepted: 21 May 2010
  •  Published: 13 June 2011


Molasses was used as a sole carbon source for the protease production fromBacillus subtilis EFRL 01 in batch wise submerged condition. The bacterial culture was grown on mineral medium and maximum production was noted after 8 h of incubation. The effect of different variable such as carbon sources (0.5 and 1.0%), nitrogen sources (0.75), sodium chloride, potassium chloride, zinc chloride (0.5 - 3.0%), pH (3 - 12) and temperature (25 - 55°C) on the protease production was checked. The maximum enzyme production was noted when B. subtilis EFRL 01 was grown on mineral medium containing 1.0% molasses, 0.75% peptone and 2.0% sodium chloride when incubated at 45°C for 8 h with initial pH 8.5. The enzyme produced by B. subtilis is pH stable and thermostable, which can be utilized in local detergent and leather industry.


Key words: Bacillus subtilis EFRL 01, molasses, protease.