African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Efficient micropropagation of Epidendrum secundum Jacq. from leaves and protocorms

Danielle Lopes Ferreira
  • Danielle Lopes Ferreira
  • Departament of Botany, Federal University of Parana, Av. Coronel Francisco Hofman dos Santos, s/n, Jardim das Americas, 81531-970. CP 19031 Curitiba, PR, Brazil.
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Eric de Camargo Smidt
  • Eric de Camargo Smidt
  • Departament of Botany, Federal University of Parana, Av. Coronel Francisco Hofman dos Santos, s/n, Jardim das Americas, 81531-970. CP 19031 Curitiba, PR, Brazil.
  • Google Scholar
Luciana Lopes Fortes Ribas*
  • Luciana Lopes Fortes Ribas*
  • Departament of Botany, Federal University of Parana, Av. Coronel Francisco Hofman dos Santos, s/n, Jardim das Americas, 81531-970. CP 19031 Curitiba, PR, Brazil.
  • Google Scholar


  •  Received: 03 February 2015
  •  Accepted: 25 March 2015
  •  Published: 01 April 2015

Abstract

An efficient method for the large-scale propagation of Epidendrum secundum using protocorms and leaves from in vitro germination was established. Explants were inoculated in Woody Plant Medium (WPM) supplemented with 6-benzyladenine (BA) (1.0–40.0 µM) for 60 days and cultured in a growth room in darkness (30 days) or with a 16 h photoperiod. Protocorms were most effective for regenerating protocorm-like bodies (PLBs; 100.0%), independent of the presence or absence of light. The leaves were less responsive, and the best results were obtained with the youngest leaves (58.0% PLB induction). BA influenced the responses of protocorms and young leaves, and medium supplemented with 1.0 µM BA was most effective for inducing and regenerating PLBs. The shoots were rooted (100.0%) in WPM without any growth regulator and were transplanted to Isopor trays containing Plantmax florestal® and vermiculite (2:1), with 100.0% survival after 60 days. A histological analysis demonstrated that for both the explants, leaves and protocorms, PLBs were formed from successive divisions of epidermal cells. This simple protocol will be useful for the large-scale propagation of E. secundum.
 
Key words: Orchidaceae, epiphytic orchid, in vitro propagation, micropropagation.

Abbreviation

BA, 6-Benzyladenine; PLBs, protocorm-like bodies; WPM, woody plant medium.