African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Active substance from some blue green algal species used as antimicrobial agents

Hanan M. Khairy and Hala Y. El-Kassas
Hydrobiology Department, Marine Environmental Division, National Institute of Oceanography and Fisheries, Alexandria, Egypt.
Email: [email protected]

  •  Accepted: 29 March 2010
  •  Published: 10 May 2010

Abstract

 

The concept of biological control for health maintenance has received widespread attention during the last few years. Therefore, the main objective of this work was to look for active substances that could be used as antimicrobial agents in an efficient and safe manner. To achieve this target, five different extracts (ethyl acetate, chloroform, diethyl ether, methanol and water) from three blue green algal species (Anabaena flos aquae (Linnaeus) Bory; Anabaena variabilis (Kützing) andOscillatoria angustissima West and West) were examined. This different algal extracts were tested in vitro for their antimicrobial effects against eight Gram +ve and Gram -ve bacteria in addition to two groups of fungi (filamentous fungi and yeast) using agar well diffusion method. The results showed that the ethyl acetate extract of A. flos aquae notably inhibited nearly the whole tested bacteria and filamentous fungi with minimal activity against tested yeasts. Furthermore, a significant reduction in sporulation as well as dry weight of the two filamentous fungi was found with ethyl acetate extracts. The toxicity of ethyl acetate extract to Artemia salina showed that the effective concentration that would inhibit their growth by 50% (LC50) after 24 h of incubation was 17, 41 and 45 mg.ml-1 for A. flos aquae, A. variabilis and O. angustissima, respectively. The antagonistic materials from ethyl acetate extract of different cyanobacterial species were identified using gas liquid chromatography mass spectrometer (GC-MS). The main components of ethyl acetate extract of A. flos aquae consisted of heptadecane and 7-methylheptadecane representing 26.11 and 18.65%, respectively.

 

Key words: Antibacterial, antifungal, cyanobacteria, ethyl acetate extract, heptadecane.