African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12267

Full Length Research Paper

Drilling fluid base oil biodegradation potential of a soil Staphylococcus species

C. O. Nweke1 and G. C. Okpokwasili*
  Department of Microbiology, University of Port Harcourt, P. M. B. 5323, Port Harcourt, Nigeria. 1Present address: Department of Biological Sciences, Federal University of Technology, P.M.B.1526, Owerri, Nigeria.
Email: [email protected]

  •  Accepted: 11 August 2003
  •  Published: 30 September 2003

Abstract

 

Staphylococcus sp. isolated from oil-contaminated soil was grown in 1% drilling fluid base oil, HDF- 2000, as a sole source of carbon and energy. The organism has strong affinity for the substrate, growing at the rate of 0.16 h-1. It uses adherence and emulsification as mechanisms for oil uptake. In a nutrient-rich marine broth, base oil (up to 2.0% v/v) and glucose (up to 1.6% w/v) have no significant effect on the growth rates. This showed that the Staphylococcus sp. is a strong primary utilizer of the base oil and has potential for application in bioremediation processes involving oil-based drilling fluids.

 

Key words: Drilling fluid base oil, Staphylococcus sp., biodegradation.