African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Response of chickpea (Cicer arietinum L.) to inoculation with native and exotic Mesorhizobium strains in Southern Ethiopia

Wondwosen Tena
  • Wondwosen Tena
  • School of Plant and Horticultural Sciences, Hawassa University, P. O. Box, 05, Hawassa, Ethiopia.
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Endalkachew Wolde-Meskel
  • Endalkachew Wolde-Meskel
  • International Livestock Research Institute, P. O. Box 5689, Addis Ababa, Ethiopia.
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Fran Walley
  • Fran Walley
  • Department of Soil Science, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, Canada.
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  •  Received: 21 October 2015
  •  Accepted: 12 August 2016
  •  Published: 31 August 2016


A series of pot and two consecutive crop-year field experiments were conducted from 2011 to 2012 in Southern Ethiopia to determine the effectiveness of Mesorhizobium strains on two cultivars of chickpea (Shasho and Nattoli). The eight treatments included: Six rhizobial inoculants, the four best indigenous strains (Cp8, Cp41, Cp97 and Cp105); CpNSTC (National Soils Testing Center inoculant); and CpSK (Canadian inoculant), Nitrogen fertilizer and a control. The results from the field and pot experiments indicated that chickpea crop yield can be improved using proper Mesorhizobium inoculation. Inoculation had a pronounced effect on grain yield, yield component, total N uptake, grain protein content, percentage N derived from the atmosphere (%Ndfa) for the seed, and amount of seed N fixed compared to non-inoculated treatments. In the pot experiment, significant difference was recorded among the mesorhizobial strains used with the indigenous strain Cp41 highly effective in shoot dry weight (41%) mg-1 plant, grain yield (50%), total N uptake (117%), and %Ndfa (67.9%) followed by CpSK, Cp8 and Cp97. In the second crop-year field experiment, the indigenous Mesorhizobium strain Cp41 also proved highly effective in-nodule dry weight (786%) mg-1 plant, grain yield (66%), total N uptake (100%), and %Ndfa (53.7%). The maximum seed protein content was recorded during the second crop-year field experiment in Cp41 (20%), followed by N fertilizer added treatment and CpSK (18%). The chickpea indigenous rhizobial strain Cp41, was superior inoculant for almost all parameters. Thus, there are potential advantages to be gained from using efficient rhizobial inoculants under rain fed conditions in Ethiopia.


Key words: Chickpea, rhizobia inoculants, nodulation, growth, yield.