African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Extraction, quantification and degree of polymerization of yacon (Smallanthus sonchifolia) fructans

Ellem Waleska Nascimento da Fonseca Contado
  • Ellem Waleska Nascimento da Fonseca Contado
  • Chemistry Department, Biochemistry Laboratory, Federal University of Lavras ? UFLA, PO Box 3037, Zip Code 37200.000, Lavras, MG, Brazil.
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Estela de Rezende Queiroz
  • Estela de Rezende Queiroz
  • Chemistry Department, Biochemistry Laboratory, Federal University of Lavras ? UFLA, PO Box 3037, Zip Code 37200.000, Lavras, MG, Brazil.
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Denise Alvarenga Rocha*
  • Denise Alvarenga Rocha*
  • Chemistry Department, Biochemistry Laboratory, Federal University of Lavras ? UFLA, PO Box 3037, Zip Code 37200.000, Lavras, MG, Brazil.
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Rodrigo Martins Fraguas
  • Rodrigo Martins Fraguas
  • Chemistry Department, Biochemistry Laboratory, Federal University of Lavras ? UFLA, PO Box 3037, Zip Code 37200.000, Lavras, MG, Brazil.
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Anderson Assaid Simao
  • Anderson Assaid Simao
  • Chemistry Department, Biochemistry Laboratory, Federal University of Lavras ? UFLA, PO Box 3037, Zip Code 37200.000, Lavras, MG, Brazil.
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Lucimara Nazare Silva Botelho
  • Lucimara Nazare Silva Botelho
  • Chemistry Department, Biochemistry Laboratory, Federal University of Lavras ? UFLA, PO Box 3037, Zip Code 37200.000, Lavras, MG, Brazil.
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Adneia de Fatima Abreu
  • Adneia de Fatima Abreu
  • Chemistry Department, Biochemistry Laboratory, Federal University of Lavras ? UFLA, PO Box 3037, Zip Code 37200.000, Lavras, MG, Brazil.
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Mariana Aparecida Braga Celeste Maria Patto de Abreu
  • Mariana Aparecida Braga Celeste Maria Patto de Abreu
  • Chemistry Department, Biochemistry Laboratory, Federal University of Lavras ? UFLA, PO Box 3037, Zip Code 37200.000, Lavras, MG, Brazil.
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  •  Received: 20 March 2015
  •  Accepted: 25 May 2015
  •  Published: 27 May 2015

Abstract

Yacon tubers have been a distinguished alternative of fructans, drawing the attention of researchers and food industries. Since fructans are carbohydrate reserves storage can reduce their contents. Additionally, the type of extraction used can provide a higher yield of fructans. Therefore, it was necessary to study yacon storage and its influence on the extraction and quantification of fructans. Thus, the objective of this study was to evaluate three fructan extractions (water 75°C, water 95°C, ethanol 90°C) in yacon with 3 sizes (large, medium, small), stored for 20 days, at room temperature as well as to compare two quantification techniques. The three extractions can be used when fructans are quantified by high performance liquid chromatography (HPLC). For quantification by spectrometry, the best extraction method was ethanol at 90°C. Medium and small-sized tubers presented the highest contents of fructans that large tubers, and storage negatively influenced these contents. Fructan quantification by HPLC was higher than the spectrophotometric technique. All treatments showed a degree of polymerization in the range from 3 to 7, allowing numerous technological applications for fructans present in yacon.

 

Key words: Fructooligosaccharides, storage, tuber size.

Abbreviation

FOS, Fructooligosaccharides; HPLC, high performance liquid chromatography.