African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

Sapucaia nuts (Lecythis pisonis) modulate the hepatic inflammatory and antioxidant metabolism activity in rats fed high-fat diets

Marcos Vidal Martins
  • Marcos Vidal Martins
  • Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Brazil.
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Izabela Maria Montezano de Carvalho
  • Izabela Maria Montezano de Carvalho
  • Departamento de Nutrição, Universidade Federal de Sergipe, Brazil.
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Mônica Maria Magalhães Caetano
  • Mônica Maria Magalhães Caetano
  • Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Brazil.
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Renata Celi Lopes Toledo
  • Renata Celi Lopes Toledo
  • Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Brazil.
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Antônio Avelar Xavier
  • Antônio Avelar Xavier
  • Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Brazil.
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José Humberto de Queiroz
  • José Humberto de Queiroz
  • Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Brazil.
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  •  Received: 01 April 2016
  •  Accepted: 18 May 2016
  •  Published: 22 June 2016

Abstract

Lecythis pisonis Cambess is commonly known as “sapucaia” nut. Previous studies show that it is rich in unsaturated fatty acids and in antioxidant minerals. The aim of the present study was to assess the antioxidant and anti-inflamatory effects of this nut after its introduction into a control (AIN-93G) or high-fat diet in Wistar rats. The animals were divided into four groups: a control diet, the same control diet supplemented with sapucaia nuts, a high-fat diet or the high-fat diet supplemented with sapucaia nuts and were fed with these diets for 14 or 28 days. The gene expression of the markers tumor necrosis factor (TNF)-α NFκB (p65) zinc superoxide dismutase (ZnSOD) and heat shock protein 72 (HSP72) was determined by the chain reaction to the quantitative reverse transcription-polymerase chain reaction (q-PCR). The antioxidant activity was also measured as thiobarbituric acid reactive substances (TBARS) through the activity of the SOD enzyme. The groups treated with “sapucaia” nuts presented reduced lipid peroxidation values and increased ZnSOD and HSP72 gene expression, as well as decreased TNF-α and NFκB (p65) gene expression levels. The significant results showed that “sapucaia” could serve as a potential source of antioxidants and as a protector agent for the examined animals.

Key words: Sapucaia nuts, inflammation, oxidative stress, gene expression.