African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12392

Full Length Research Paper

Genetic diversity in Radix species from the middle and south of Iraq based on simple sequence repeats

Ali BM Al-Waaly
  • Ali BM Al-Waaly
  • Department of Biology, College of Science, Al-Qadisiya University, Al-Diwaniya, Iraq.
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Kanokporn Triwitayakorn
  • Kanokporn Triwitayakorn
  • Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom, Thailand.
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Nattaya Srisawad
  • Nattaya Srisawad
  • Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom, Thailand.
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Duncan R Smith
  • Duncan R Smith
  • Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom, Thailand.
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Mohammad K Mohammad
  • Mohammad K Mohammad
  • Iraq Natural History Museum, University of Baghdad, Bab Al-Muadham, Baghdad, Iraq.
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Supajit Sraphet
  • Supajit Sraphet
  • Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom, Thailand.
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  •  Received: 09 July 2018
  •  Accepted: 15 August 2018
  •  Published: 05 September 2018

Abstract

Ten newly developed microsatellite loci were isolated from an AG20 and CAG20 enriched genomic DNA library of Radix sp. from Iraq. The simple sequence repeats (SSR) comprised 76.0% di-, 20.0% tri- and 4.0% tetra-nucleotide repeats. The number of alleles for all 10 loci ranged from 4 to 18, with a mean of 11.3 alleles per locus. The observed (Ho) and expected heterozygosity (He) values varied from 0.286 to 0.8670.927 (mean 0.640) and from 0.263 to 0.939 (mean 0.753), respectively. The polymorphic information content (PIC) values ranged from 0.336 to 0.923 (mean 0.749). Five loci showed significant deviation from Hardy-Weinberg equilibrium (HWE) and no significant linkage disequilibrium (LD) was observed. Cross transferability was successfully tested across on species from the genus Melanopsis. These polymorphic SSRs will be useful for assessment of the genetic diversity and population genetics of a Radix sp. as well as other related species in Iraq.
 
Key  words: Cross-transferability, eenriched genomic DNA library, ffreshwater snail, microsatellitemicrosatellite.