African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Genetic variation within and between three Vietnamese pine populations (Pinus merkusii) using random amplified polymorphic DNA (RAPD) markers

Ho Manh Tuong*
  • Ho Manh Tuong*
  • Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
  • Google Scholar
Nguyen Thu Giang
  • Nguyen Thu Giang
  • Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
  • Google Scholar
Chu Hoang Ha
  • Chu Hoang Ha
  • Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
  • Google Scholar
Le Van Son
  • Le Van Son
  • Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
  • Google Scholar


  •  Received: 26 February 2016
  •  Accepted: 13 July 2016
  •  Published: 27 July 2016

Abstract

Pinus merkusii is an important species in Vietnam with many economic and biological contributions. The information on diversity within and between populations of a species is necessary for plantation programs, breeding and conservation strategies. Genetic diversity of three Vietnamese populations (NA, QB and QN) was analyzed using the random amplified polymorphic DNA (RAPD) markers. Nine RAPD primers produced 82 markers, 77 of which were polymorphic with 93.9% of polymorphism. The results showed higher genetic variation within populations (72%) than between populations (28%) and low Nei’s genetic differentiation index among populations (0.1867). The populations also clustered based on PCoA analysis where cluster I included NA and QB populations and Cluster II, the QN population. These results suggest that P. merkusii populations in Vietnam is necessary to develop the genetic resources.

Key words: DNA markers, genetic diversity, Pinus merkusii, random amplified polymorphic DNA (RAPD), Vietnam.