African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12240

Full Length Research Paper

Development of a rice yellow mottle virus amplicon biotechnological tool for the production of high value proteins in rice

Pingdwende Kader Aziz Bamogo
  • Pingdwende Kader Aziz Bamogo
  • IRD, CIRAD, Université Montpellier, Interactions Plantes Microorganismes et Environnement (IPME), 911 Avenue Agropolis BP 64501, 34394 Montpellier Cedex 5, France.
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Christophe Brugidou
  • Christophe Brugidou
  • IRD, CIRAD, Université Montpellier, Interactions Plantes Microorganismes et Environnement (IPME), 911 Avenue Agropolis BP 64501, 34394 Montpellier Cedex 5, France.
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Fidèle Tiendrébéogo
  • Fidèle Tiendrébéogo
  • Institut de L’Environnement et de Recherches Agricoles (INERA)/LMI Patho-Bios, Laboratoire de Virologie et de Biotechnologies Végétales, 01 BP 476, Ouagadougou 01, Burkina Faso.
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Drissa Sérém
  • Drissa Sérém
  • Institut de L’Environnement et de Recherches Agricoles (INERA)/LMI Patho-Bios, Laboratoire de Virologie et de Biotechnologies Végétales, 01 BP 476, Ouagadougou 01, Burkina Faso.
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Florencia Wendkuuni Djigma
  • Florencia Wendkuuni Djigma
  • Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE), Ecole Doctorale Sciences et Technologie, Centre de Recherche Biomoléculaire Piétro Annigoni (CERBA), Université Joseph Ki-Zerbo, BP 364, Ouagadougou 01, Burkina Faso.
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Jacques Simpore
  • Jacques Simpore
  • Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE), Ecole Doctorale Sciences et Technologie, Centre de Recherche Biomoléculaire Piétro Annigoni (CERBA), Université Joseph Ki-Zerbo, BP 364, Ouagadougou 01, Burkina Faso.
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Séverine Lacombe
  • Séverine Lacombe
  • IRD, CIRAD, Université Montpellier, Interactions Plantes Microorganismes et Environnement (IPME), 911 Avenue Agropolis BP 64501, 34394 Montpellier Cedex 5, France.
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  •  Received: 06 November 2019
  •  Accepted: 29 November 2019
  •  Published: 31 December 2019

Abstract

Lately, advances on biotechnology have led to the emergence of plants as bioreactor for the production of molecules of industrial, pharmaceutical and agribusiness interests. One of the major advances was the exploitation of plant viruses as “amplifier” for the protein production. This amplicon technology relies on viral vector engineered to contain a gene encoding candidate protein that is produced in significant quantities during virus replication. To date, only few of these virus tools are developed for monocot species. Here, we set up experimental approaches to develop the first amplicon tool based on the Rice yellow mottle virus (RYMV) to be used on rice. We showed that inoculated 4-week-old leaves display the most important RYMV initial replication and retained this tissue for protein of interest production from RYMV amplicon. We engineered RYMV tool based on highly replicative RYMVMg1 isolated with GFP gene of interest replacing Coat Protein ORF. Replication of RYMV amplicon was optimized in inoculated rice leaves by adding an RYMV helper to complement RYMV amplicon with CP. This work identify for the first time a viral amplicon tool active in rice offering promising perspectives for the use of rice as bioreactor for the production of high value proteins.
 
Key words: Rice, rice yellow mottle virus, plant bioreactor, viral amplicon, virus induced gene silencing (VIGS).