African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12368

Full Length Research Paper

The effects of reciprocal cross on inheritance of DNA methylation in cotton (Gossypium hirsutum)

  Jun Wei1#, Honghong Fan1#, Tingchun Li1,2, Wanghua Wang, Ning Guo, Zhengpeng Li1, Yongping Cai1 and Yi Lin1*        
  1Department of Life Science, Anhui Agricultural University, Hefei, 230036, Peoples Republic of China. 2 Anhui Academy of Agricultural Sciences, Hefei, 230031, Peoples Republic of China.
Email: [email protected]

  •  Accepted: 23 February 2012
  •  Published: 20 March 2012

Abstract

 

DNA methylation plays an important role for regulation of gene expression. To study the inheritance of DNA methylation, we selected two Fplant population by reciprocal cross with two cotton lines Zongcaixuan No.1 and HY428, and analyzed the variations of DNA methylation levels and patterns in Fgenerations by methylation sensitive amplified fragment length polymorphism (MSAP) technique with 54 primer combinations. The results show that most cytosine methylated patterns are conservatively inherited from parents. The numbers of variant sites are less in F1generation. According to the number of sites individually inherited from female and male parents, the different choice of female and male parents made a big distinction between the sites inherited from female parent and the sites inherited from the male parent. For reciprocal cross F113 and F132, the number for sites of cytosine methylation patterns inherited from the female parent are far more than that from the male parent, which may be closely related to cytoplasmic inheritance.

 

Key words: Methylation sensitive amplified fragment length polymorphism (MSAP), DNA methylation, cytoplasmic inheritance, reciprocal cross, cotton.

Abbreviation

 

 MSAP, Methylation sensitive amplified fragment length polymorphism.

 

#These authors contributed equally to this work.