African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Control of lethal browning by using ascorbic acid on shoot tip cultures of a local Musa spp. (Banana) cv. Mzuzu in Tanzania

Munguatosha Ngomuo1
  • Munguatosha Ngomuo1
  • School of Life Sciences, Nelson Mandela African Institute of Science and Technology, P.O. Box 447, Arusha, Tanzania.
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Emerald Mneney
  • Emerald Mneney
  • Mikocheni Agricultural Research Institute, P.O. Box 6226, Dar es salaam, Tanzania.
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Patric Ndakidemi*
  • Patric Ndakidemi*
  • School of Life Sciences, Nelson Mandela African Institute of Science and Technology, P.O. Box 447, Arusha, Tanzania.
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  •  Received: 11 September 2013
  •  Accepted: 25 March 2014
  •  Published: 16 April 2014

Abstract

The use of ascorbic acid during explants preparation and the effect of different concentrations of ascorbic acid in controlling lethal browning and survival of the explants in local banana cv. Mzuzu banana were investigated. The explants were taken from young suckers. The shoot tips were cultured on Murashige and Skoog’s media supplemented with 5 mg/l of benzylaminopurine (BAP) and different concentrations of ascorbic acid (0, 50, 100 and 200 mg/l). Completely randomized design was used in this study. The results indicate that the use of ascorbic acid as an antioxidant during explants preparation significantly reduced the extent of lethal browning and survival of the explants followed by 100 mg/l of ascorbic acid applied directly into the media.

 

Key words: Micro propagation, surface sterilization, survival of explants, tissue culture.

Abbreviation

Abbreviations: MS, Murashige and Skoog (1962); AA, ascorbic acid; BAP, benzylaminopurine; AC, activated charcoal.