African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

In vitro methods for mutation induction in potato (Solanum tuberosum L.)

Souleymane Bado
  • Souleymane Bado
  • Plant Breeding and Genetics Laboratory (PBGL), Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA, P.O. Box 100, Wagramerstrasse 5, A-1400 Vienna, Austria.
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Matumelo Alice Rafiri
  • Matumelo Alice Rafiri
  • Department of Agricultural Research, Horticulture Section, P.O. Box 829, Maseru 100, Lesotho.
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Kaoutar El-Achouri
  • Kaoutar El-Achouri
  • Institut National de Recherche Agronomique, Centre de Tanger, Tanger 90010, Morocco.
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Enoch Sapey
  • Enoch Sapey
  • Council for Scientific and Industrial Research (CSIR)-Oil Palm Research Institute (OPRI) P.O. Box 78, Kade, Ghana.
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Stephan Nielen
  • Stephan Nielen
  • Plant Breeding and Genetics Laboratory (PBGL), Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA, P.O. Box 100, Wagramerstrasse 5, A-1400 Vienna, Austria.
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Abdelbagi Mukhtar Ali Ghanim
  • Abdelbagi Mukhtar Ali Ghanim
  • Plant Breeding and Genetics Laboratory (PBGL), Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA, P.O. Box 100, Wagramerstrasse 5, A-1400 Vienna, Austria.
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Brian Peter Forster
  • Brian Peter Forster
  • Plant Breeding and Genetics Laboratory (PBGL), Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA, P.O. Box 100, Wagramerstrasse 5, A-1400 Vienna, Austria.
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Margit Laimer
  • Margit Laimer
  • Plant Biotechnology Unit, Department of Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, Austria.
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  •  Received: 18 July 2016
  •  Accepted: 07 September 2016
  •  Published: 28 September 2016

Abstract

Potato (Solanum tuberosum L.) is an important vegetable and staple crop worldwide and mainly propagated vegetatively. Breeding of potato is problematic and therefore induced mutation is an attractive means of improving the crop. In vitro culture systems, and especially the production of micro-tubers, are ideal for such purposes in potato improvement. Radio-sensitivity testing (growth reduction, GR and lethal dose, LD) allows the determination of irradiation treatments (Gy) for mutation induction. Three schemes incorporating in vitro techniques were tested for mutation induction in potato namely: 1) irradiation of cuttings without leaves and subsequent dissociation of chimeras to produce plantlets or micro-tubers on M1V2 (or further generation) plantlets, 2) irradiation of cuttings with leaves and direct induction of mutant micro-tubers, and 3) induction and irradiation of micro-tubers. Variability among the potato genotypes to gamma irradiation was recorded. Optimized irradiation treatments for mutation induction were established for the various tissues/propagules: cutting growth (GR50, 9-6 to 20.6 Gy), cutting tuberization ability (LD50, 7.3 to 13 Gy) and micro-tuber sprouting ability (LD50, 20.6 to 54.8 Gy). Micro-tubers were found to be more resistant for in vitro mutation induction than in vitro cuttings. This study shows the susceptibility of different plant tissue/propagule and potato genotypes to gamma irradiation. Radio-sensitivity analyses showed that lower gamma doses are required when mutation induction is applied in combination with micro-tuberization.

 

Key words: Potato, gamma irradiation, stem cuttings, micro-tubers, in vitro tuberization.