African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12172

Full Length Research Paper

Cellulase production and molecular identification of Xanthobacter flavus (IM32-90) isolated under eucalyptus plantation in the cerrado of Minas Gerais

Farley Souza Ribeiro Menezes
  • Farley Souza Ribeiro Menezes
  • Institute of Technological Sciences, Federal University of the Valleys of Jequitinhonha and Mucuri, Brazil.
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Maria Cecília Mota Docha
  • Maria Cecília Mota Docha
  • Department of Forest Engineering, Faculty of Agrarian Sciences, Federal University of the Valleys of Jequitinhonha and Mucuri, Brazil.
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Any Caroliny Pinto Rodrigues
  • Any Caroliny Pinto Rodrigues
  • Department of Forest Engineering, Faculty of Agrarian Sciences, Federal University of the Valleys of Jequitinhonha and Mucuri, Brazil.
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Jéssica Naiara Dos Santos
  • Jéssica Naiara Dos Santos
  • Department of Forest Engineering, Faculty of Agrarian Sciences, Federal University of the Valleys of Jequitinhonha and Mucuri, Brazil.
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Gizelly Gomes da Cruz
  • Gizelly Gomes da Cruz
  • Institute of Technological Sciences, Federal University of the Valleys of Jequitinhonha and Mucuri, Brazil.
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Viviane Ferreira dos Anjos
  • Viviane Ferreira dos Anjos
  • Department of Forest Engineering, Faculty of Agrarian Sciences, Federal University of the Valleys of Jequitinhonha and Mucuri, Brazil.
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Tarcisio Tomás Cabral de Sousa
  • Tarcisio Tomás Cabral de Sousa
  • Department of Forest Engineering, Faculty of Agrarian Sciences, Federal University of the Valleys of Jequitinhonha and Mucuri, Brazil.
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Marcelo Luiz de Laia
  • Marcelo Luiz de Laia
  • Department of Forest Engineering, Faculty of Agrarian Sciences, Federal University of the Valleys of Jequitinhonha and Mucuri, Brazil.
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  •  Received: 07 February 2019
  •  Accepted: 10 April 2019
  •  Published: 24 April 2019

Abstract

Lignocellulosic biomass has become a major ally for production of biofuels as a clean and renewable energy source. The manufacturing of cellulolytic enzymes capable of hydrolyzing lignocellulose and producing fermentable sugars has been, and still is a great challenge. There is a wide variety of microorganisms, both bacteria and fungi, capable of producing cellulolytic enzymes. These microorganisms can be grown by solid state fermentation (SSF) or submerged fermentation (FS). In this study, the production of endoglucanase and β-glycosidase by the bacterium IM32-90 were investigated using a 168-h submerged fermentation. The DNA was extracted, purified and subjected to molecular identification, and IM32-90 was classified as a Xanthobacter flavus species. The bacterium IM32-90 produces cellulolytic enzymes, with activities of 0.214 U.mL-1 for endoglucanase and 0.056 U.mL-1 for β-glucosidase. 
 
Key words: Bacteria, biofuels, cellulase, molecular, renewable sources.