African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12218

Full Length Research Paper

Adoption of pelleted Digitalia iburua grain as carrier for heat stable Newcastle disease vaccine virus for village poultry

Ibu, O. J.
  • Ibu, O. J.
  • Department of Veterinary Pathology and Microbiology, University of Agriculture, Makurdi, Nigeria.
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Shittu, A. I.
  • Shittu, A. I.
  • Viral Vaccine Research Division, National Veterinary Research Institute, Vom, Plateau, Nigeria.
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Egbuji, A.
  • Egbuji, A.
  • Viral Vaccine Research Division, National Veterinary Research Institute, Vom, Plateau, Nigeria.
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Okoye, J. O. A.
  • Okoye, J. O. A.
  • Department of Veterinary Microbiology, University of Nigeria, Nsukka, Enugu, Nigeria.
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Echeonwu, G. O. N.
  • Echeonwu, G. O. N.
  • College of Veterinary and Medical Laboratory Technology, Vom, Plateau, Nigeria.
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Abdu, P.
  • Abdu, P.
  • Department of Veterinary Medicine, Ahamadu Bello University, Zaria, Nigeria.
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Okwor, E. C.
  • Okwor, E. C.
  • Department of Veterinary Microbiology, University of Nigeria, Nsukka, Enugu, Nigeria.
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Eze, D.
  • Eze, D.
  • Department of Veterinary Microbiology, University of Nigeria, Nsukka, Enugu, Nigeria.
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Usman, M.
  • Usman, M.
  • Faculty of Veterinary Medicine, Usman Danfodio University, Sokoto, Nigeria
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Lohlum, A.
  • Lohlum, A.
  • Biochemistry and Molecular Biology Division, National Veterinary Research Institute, Vom, Plateau, Nigeria.
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Rabo, J. S.
  • Rabo, J. S.
  • Department of Veterinary Pathology and Microbiology, University of Agriculture, Makurdi, Nigeria.
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  •  Received: 20 March 2017
  •  Accepted: 30 May 2017
  •  Published: 18 October 2017

Abstract

The development of pelleted food–based vaccine against Newcastle disease (ND) using a thermostable NDVI2 strain in Acha (Digitalia iburua) is reported. The Acha cereal was subjected to rigorous processing of washing, soaking, boiling, roasting and grinding into fine powder prior to virus incorporation. The mixture which was passed through a locally fabricated pelleting machine resulted in fine pellets. The virus content in the pellets determined by inoculation of susceptible embryonated chicken eggs showed appreciable titres above 108 Egg Infective Dose 50% end points (EID50) per g of feed. These titres appear to be higher than the recommended minimum immunizing dose of 105.5 EID50 per ml of vaccine. Further in-vitro and in-vivo assessment of the pelleted vaccine is advocated.

 

Key words: Pelleted, NDVI2, food vaccine, Digitalia iburua, high virus titres.