African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12196

Full Length Research Paper

Cloning and expression of acyl homoserine lactone (AHL) lactonase genes of Bacillus cereus INT1c and Bacillus thuringiensis SGT3g in Escherichia coli

Iman Rusmana
  • Iman Rusmana
  • Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Jalan Agatis, Dramaga, Bogor, 16680, West Java, Indonesia.
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Anja Asmarany
  • Anja Asmarany
  • Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Jalan Agatis, Dramaga, Bogor, 16680, West Java, Indonesia.
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Aris Tri Wahyudi
  • Aris Tri Wahyudi
  • Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Jalan Agatis, Dramaga, Bogor, 16680, West Java, Indonesia.
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  •  Received: 11 August 2017
  •  Accepted: 15 September 2017
  •  Published: 20 September 2017

Abstract

Inhibition of virulence genes expression of phytopathogenic bacteria can be done by degrading the acyl-homoserine lactone (AHL) compounds using AHL-lactonase. In this study, the AHL-lactonase genes were cloned and expressed from Bacillus cereus INT1c and Bacillus thuringiensis SGT3g in Escherichia coli, in order to understand the characteristics and biocontrol mechanisms of their AHL-lactonase. Amplification using aiiA primers succeeded to get 800 bp DNA fragments of AHL-latonase genes having a complete open reading frame (ORF) and encoding 250 amino acids. AHL-lactonase genes of B. cereus INT1c and B. thuringiensis SGT3g were expressed in E. coli BL21 (DE3) plasmid with T7 lysozyme coding sequence (pLysS). AHL-lactonase of the isolates is classified as metallo-β-lactamase superfamily domain and the AHL-lactonase inhibited violacein production of Chromobacterium violaceum.

Key words: Acyl-homoserine lactone (AHL), lactonase, aiiA gene, cloning, Bacillus thuringiensis, Bacillus cereus.