African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12191

Full Length Research Paper

Surface sterilant effect on the regeneration efficiency from cotyledon explants of groundnut (Arachis hypogea L.) varieties adapted to eastern and Southern Africa

Susan Muthoni Maina1, Quinata Emongor2, Kiran K. Sharma3, Simon T. Gichuki2, Moses Gathaara1 and Santie M. de Villiers4*
  1Kenyatta University PO Box 43844 - 00100 Nairobi, Kenya. 2Kenya Agricultural Research Institute (KARI) Biotechnology Centre PO Box 57811, Nairobi, Kenya. 3International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Andhra Pradesh 502 324, India. 4International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Nairobi, PO Box 39063 - 00623, Nairobi, Kenya.
Email: [email protected]

  •  Accepted: 01 April 2010
  •  Published: 17 May 2010

Abstract

 

Five groundnut (Arachis hypogea L.) varieties - ICGV-12991, ICGV-99568, ICGV-90704, CG-2 and Chalimbana - that are adapted to Eastern and Southern Africa were compared to variety JL 24 for their regeneration response in tissue culture. Sodium hypochlorite and mercuric chloride were compared for efficiency as sterilizing agents and subsequent effect on regeneration. All five varieties formed shoot buds that elongated well on shoot elongation medium. ICGV-90704 and Chalimbana performed better than the other three varieties for shoot organogenesis although all varieties produced healthy rooted plants in vitro that were successfully transferred to the greenhouse where they exhibited normal growth, flowering and seed set. Both sterilizing agents were suitable, but mercuric chloride was less harmful than sodium hypochlorite. This study established a basis for genetic engineering activities on African groundnuts in the future.

 

Key words: Groundnuts, regeneration, genotype independent, Eastern and Southern Africa.